Document Detail

Distribution of type II collagen mRNA in Xenopus embryos visualized by whole-mount in situ hybridization.
MedLine Citation:
PMID:  1619277     Owner:  NLM     Status:  MEDLINE    
We have developed a whole-mount histochemical method to monitor the distribution of expressed genes within the intact, developing vertebrate embryo. Background problems that result from alkaline phosphatase- or horseradish peroxidase-based stains have been minimized, enabling both early and late stages of Xenopus embryogenesis to be monitored. The feasibility and utility of this non-isotopic method has been demonstrated by using a specific DNA probe to localize Xenopus laevis Type II collagen mRNA expression to areas surrounding the vacuoles of the notochord in Stage 30 embryos. Expression expands by Stage 41/42 to form a visually striking distribution pattern that includes a variety of chondrogenic tissues such as the vertebrae, otocysts, mandible, and periocular region. Although these experiments focused on expression of a structural gene, the high resolution and sensitivity of the method should allow it also to monitor expression of less abundant mRNA products of non-structural genes such as transcription factors, cytoplasmic regulators, and growth factors. In addition, this approach should be a successful tool to probe expression in normal and perturbed embryos not only of amphibians but also of other vertebrates, including avians and mammals.
J J Bieker; M Yazdani-Buicky
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society     Volume:  40     ISSN:  0022-1554     ISO Abbreviation:  J. Histochem. Cytochem.     Publication Date:  1992 Aug 
Date Detail:
Created Date:  1992-08-06     Completed Date:  1992-08-06     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9815334     Medline TA:  J Histochem Cytochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1117-20     Citation Subset:  IM    
Brookdale Center for Molecular Biology, Mount Sinai School of Medicine, New York, New York 10029-6574.
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MeSH Terms
Collagen / biosynthesis,  genetics*
DNA Probes
Embryo, Nonmammalian / chemistry*,  metabolism
Gene Expression*
Notochord / chemistry,  metabolism
Nucleic Acid Hybridization*
RNA, Messenger / analysis*,  genetics
Xenopus laevis
Grant Support
Reg. No./Substance:
0/DNA Probes; 0/RNA, Messenger; 9007-34-5/Collagen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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