Document Detail

Distribution and stability of aflatoxin M1 during processing, ripening and storage of Telemes cheese.
MedLine Citation:
PMID:  11358185     Owner:  NLM     Status:  MEDLINE    
Telemes cheeses were produced using milk that was artificially-contaminated with aflatoxin M1 at the levels of 0.050 and 0.100 microg/l. The cheeses produced in the two cheese-making trials were allowed to ripen for 2 months and stored for an additional 4 months to simulate commercial production of Telemes cheese. Concentrations of aflatoxin M1 in whey, curd, brine, and the produced cheeses were determined at intervals by liquid chromatography and fluorometric detection coupled with immunoaffinity column extraction. Concentrations of aflatoxin M1 in the produced curds were found to be 3.9 and 4.4 times higher than those in milk, whereas concentrations in whey were lower than those in curd and milk. Aflatoxin M1 was present in cheese at higher concentrations at the beginning than at the end of the ripening/storage period, and it declined to concentrations 2.7 and 3.4 times higher than those initially present in milk by the end of the sixth month of storage. Concentrations of aflatoxin M1 in brine started low and increased by the end of the ripening/storage period but only a portion of the amounts of aflatoxin M1 lost from cheese was found in the brine. Results showed that Telemes cheeses produced from milk containing aflatoxin M1 at a concentration close to either the maximum acceptable level of 0.05 microg/l set by the European union (EU) or at double this value, will contain the toxin at a level that is much lower or slightly higher, respectively, than the maximum acceptable level of 0.250 microg of aflatoxin M1/kg cheese set by some countries.
A Govaris; V Roussi; P A Koidis; N A Botsoglou
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Food additives and contaminants     Volume:  18     ISSN:  0265-203X     ISO Abbreviation:  Food Addit Contam     Publication Date:  2001 May 
Date Detail:
Created Date:  2001-05-18     Completed Date:  2001-12-07     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  8500474     Medline TA:  Food Addit Contam     Country:  England    
Other Details:
Languages:  eng     Pagination:  437-43     Citation Subset:  IM    
Laboratory of Hygiene of Foods of Animal Origin, Faculty of Veterinary Medicine, University of Thessaly, Karditsa, Greece.
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MeSH Terms
Aflatoxin M1 / analysis*,  metabolism
Carcinogens / analysis*,  metabolism
Cheese / analysis*
Chromatography, Liquid
Food Contamination / analysis*
Food Handling
Food Preservation
Milk / chemistry
Reg. No./Substance:
0/Carcinogens; 6795-23-9/Aflatoxin M1

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