Document Detail

Distribution of splicing proteins and putative coiled bodies during pollen development and androgenesis in Brassica napus L.
MedLine Citation:
PMID:  11732187     Owner:  NLM     Status:  MEDLINE    
Small nuclear ribonucleoprotein particles (snRNPs) are subunits of splicing complexes, which show a transcription-dependent localization pattern. We have analyzed the labelling pattern of snRNPs during pollen development and microspore and pollen embryogenesis in Brassica napus with an antibody which recognizes protein D of U1, U2, U4, U5, and U6 snRNPs. It was found that nuclei were labelled almost uniformly for snRNPs in microspores and young bicellular pollen. In the generative nuclei of late-bicellular pollen and in the vegetative nuclei and sperm nuclei of mature pollen no snRNPs could be detected. The snRNP-positive nuclei contained mostly one or two brightly labelled nuclear bodies, most likely coiled bodies, often closely related to the nucleolus. These nuclear bodies increased in size from 0.5 micron in nuclei of young microspores up to 2 microns in nuclei of late microspores and the vegetative nucleus of early-bicellular pollen. Also their number increased during these developmental stages. After induction of embryogenesis the size of the coiled bodies decreased to about 0.5 micron and in several occasions the coiled body was found free in the nucleoplasm, away from the nucleolus. The results support the idea that the size and number of coiled bodies coincide with changes in general nuclear activity. They also indicate that, in nuclei of Brassica napus, at least assembly and disassembly of coiled bodies takes place in the nucleoplasm, whereas mature coiled bodies are located adjacent to the nucleolus.
K R Straatman; J H Schel
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Protoplasma     Volume:  216     ISSN:  0033-183X     ISO Abbreviation:  Protoplasma     Publication Date:  2001  
Date Detail:
Created Date:  2001-12-04     Completed Date:  2002-02-19     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9806853     Medline TA:  Protoplasma     Country:  Austria    
Other Details:
Languages:  eng     Pagination:  191-200     Citation Subset:  IM    
Laboratory of Plant Cell Biology, Wageningen University, Wageningen.
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MeSH Terms
Brassica napus / chemistry,  embryology,  physiology*,  ultrastructure
Cell Nucleus / metabolism*
Coiled Bodies / metabolism*
Pollen / chemistry,  growth & development*,  metabolism
Ribonucleoproteins, Small Nuclear / metabolism*
Reg. No./Substance:
0/Ribonucleoproteins, Small Nuclear

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