Document Detail


Distribution of sialoglycoconjugates in the oviductal isthmus of the horse during anoestrus, oestrus and pregnancy: a lectin histochemistry study.
MedLine Citation:
PMID:  15718207     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The distribution of sialic acid residues as well as other glycosidic sugars has been investigated in the horse oviductal isthmus during anoestrus, oestrus and pregnancy by means of lectin and pre-lectin methods. Ciliated cells and non-ciliated (secretory) cells exhibited different lectin binding profiles that were found to change during the investigated stages. Ciliated cells did not show any reactivity in the basal cytoplasm, while the supra-nuclear cytoplasm displayed a few of oligosaccharides with terminal and internal alphamannose (Man) and/or alphaglucose (Glc) during oestrus and pregnancy and a moderate presence of oligosaccharides terminating in alphafucose (Fuc) during oestrus; cilia exhibited a more complex glycoconjugate pattern for the presence of oligosaccharides terminating in N-acetylgalactosamine (GalNAc), GalNAcalpha1,3 GalNAcalpha1,3galactose(Gal)beta1,4Galbeta1,4N-acetylglucosamine(GlcNAc), Fuc, sialic acid (Neu5Ac)-aGalNAc belonging or not to the GalNAca1,3GalNAca1,3 Galb1,4 Galb1, 4GlcNAc sequence, and. alphaGalNAc and Neu5Aca 2,6Gal/GalNAc increased during oestrus. Cilia displayed terminal Galbeta1,3 GalNAc in pregnancy, terminal alphaGal in anoestrus and pregnancy and terminal or internal D-GlcNAc during anoestrus and pregnancy, respectively. The whole cytoplasm of non-ciliated cells showed oligosaccharides terminating with alphaGalNAc, Neu5Aca2,6Gal/GalNAc, Neu5Ac GalNAca 1,3GalNAcalpha1,3Galbeta1,4Galbeta1,4GlcNAc during the investigated stages, as well as GlcNAc in anoestrus and pregnancy. The supra-nuclear zone of non-ciliated cells exhibited oligosaccharides with terminal Galbeta1,4GlcNAc and internal Man during oestrus and pregnancy as well as terminal alphaGal and Fuc in oestrus and Neu5Ac-Galbeta1,3GalNAc in pregnancy. The luminal surface of non-ciliated cells showed glycans terminating with alphaGalNAc and/or Neu5Ac GalNAcalpha1,3 GalNAcalpha1,3Galbeta1,4Galbeta1,4GlcNAc in all specimens, oligosaccharides with terminal Galbeta1,4GlcNAc and internal Man during oestrus and pregnancy, Neu5Ac alpha2,6Gal/GalNAc in anoestrus and oestrus, and glycans terminating with Galbeta1,3GalNAc, Neu5A acalpha2,3 Galbeta1, 4GlcNac, Neu5ac-Galbeta1,3GalNAc, Neu5Ac-Galbeta1,4 GlcNAc in pregnancy. These findings show the presence of sialoglycoconjugates in the oviductal isthmus of the mare as well as the existence of great modifications in the glycoconjugates linked to different physiological conditions.
Authors:
S Desantis; F Acone; A Corriero; M Deflorio; D Zubani; G Ventriglia; G Palmieri; G De Metrio
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  European journal of histochemistry : EJH     Volume:  48     ISSN:  1121-760X     ISO Abbreviation:  Eur J Histochem     Publication Date:    2004 Oct-Dec
Date Detail:
Created Date:  2005-02-18     Completed Date:  2005-07-14     Revised Date:  2008-08-15    
Medline Journal Info:
Nlm Unique ID:  9207930     Medline TA:  Eur J Histochem     Country:  Italy    
Other Details:
Languages:  eng     Pagination:  403-12     Citation Subset:  IM    
Affiliation:
Dept. of Animal Health and Well-being, Faculty of Veterinary Medicine, University of Bari, Italy.
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MeSH Terms
Descriptor/Qualifier:
Anestrus*
Animals
Estrus*
Fallopian Tubes / chemistry*
Female
Glycoconjugates / analysis*,  metabolism
Horses / metabolism*
Lectins / chemistry,  metabolism
N-Acetylneuraminic Acid / analysis*,  metabolism
Pregnancy / metabolism*
Chemical
Reg. No./Substance:
0/Glycoconjugates; 0/Lectins; 131-48-6/N-Acetylneuraminic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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