| Distribution of rpoB mutations among multidrug-resistant Mycobacterium tuberculosis (MDRTB) strains from Thailand and development of a rapid method for mutation detection. | |
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MedLine Citation:
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PMID: 18294243 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Since rifampicin resistance is a surrogate marker for multidrug-resistant Mycobacterium tuberculosis (MDRTB), the present study aimed to investigate rpoB mutations conferring rifampicin resistance in M. tuberculosis strains from Thailand, and to develop a rapid, inexpensive and simple PCR-based method for rapid detection of MDRTB. Overall, 267 M. tuberculosis isolates, including 143 MDRTB isolates, were investigated. Isolates of the Beijing strain predominated among the MDRTB isolates (79.1%), but accounted for only 45.5% of the susceptible isolates. Mutations in the rpoB gene were found most commonly at codons 531, 526 and 516 (58%, 25.2% and 9.1%, respectively). A multiplex allele-specific PCR was developed and tested with 216 clinical isolates. In comparison with the proportion method, the method showed 94.2% sensitivity and 100% specificity, and had a 100% positive predictive value and a 95% negative predictive value, which suggested that this method could be useful for screening for MDRTB, particularly in resource-limited countries. |
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Authors:
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T Prammananan; W Cheunoy; D Taechamahapun; J Yorsangsukkamol; S Phunpruch; P Phdarat; M Leechawengwong; A Chaiprasert |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-02-22 |
Journal Detail:
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Title: Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases Volume: 14 ISSN: 1198-743X ISO Abbreviation: Clin. Microbiol. Infect. Publication Date: 2008 May |
Date Detail:
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Created Date: 2008-04-10 Completed Date: 2008-06-18 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9516420 Medline TA: Clin Microbiol Infect Country: France |
Other Details:
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Languages: eng Pagination: 446-53 Citation Subset: IM |
Affiliation:
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National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, Thailand Science Park, Pathumthani, Thailand. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Bacterial Proteins
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genetics* DNA Mutational Analysis / methods DNA, Bacterial / genetics* Drug Resistance, Multiple, Bacterial / genetics* Mycobacterium tuberculosis / genetics* Rifampin / pharmacology Thailand |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/DNA, Bacterial; 0/rpoB protein, Mycobacterium tuberculosis; 13292-46-1/Rifampin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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