Document Detail

Distribution of nicotinic acetylcholine receptor subunits alpha7 and beta2 in the human brainstem and hippocampal formation.
MedLine Citation:
PMID:  20566330     Owner:  NLM     Status:  MEDLINE    
We provide the first quantification and localization of the nicotinic acetylcholine receptors (nAChRs) alpha7 and beta2 in the human adult and infant brainstem and hippocampus. After applying immunohistochemistry on formalin fixed and paraffin embedded human brain tissue, we qualitatively analyzed the staining to provide a comparison in expression amongst the brainstem nuclei and hippocampal regions, and between the infants and adults. Amongst the brainstem regions studied, the greatest protein expression for both alpha7 and beta2 subunits was in the motor nuclei of the medulla and pons. Lowest expression for both subunits was in the midbrain nuclei, except for the oculomotor nucleus. Comparison between infants and adults showed greater expression in the infant brainstem nuclei of the dorsal motor nucleus of the vagus, hypoglossal, inferior olivary nucleus, nucleus of the solitary tract, abducens, and facial nuclei, for both alpha7 and beta2 nAChRs. In the hippocampus, only the alpha7 subunit showed greater expression in infants compared to adults. We conclude that both alpha7 and beta2 containing nAChRs play an important role in many nuclei of the human infant and adult brainstem, and that the alpha7 subunit may have a more prominent role in the infant than adult hippocampus.
Rita Machaalani; Petra K Kashi; Karen A Waters
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-06-04
Journal Detail:
Title:  Journal of chemical neuroanatomy     Volume:  40     ISSN:  1873-6300     ISO Abbreviation:  J. Chem. Neuroanat.     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-08-30     Completed Date:  2010-12-10     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8902615     Medline TA:  J Chem Neuroanat     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  223-31     Citation Subset:  IM    
Copyright Information:
2010 Elsevier B.V. All rights reserved.
Department of Medicine, The University of Sydney, NSW 2006, Australia.
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MeSH Terms
Brain Stem / metabolism*
Hippocampus / metabolism*
Infant, Newborn
Middle Aged
Receptors, Nicotinic / metabolism*
Reg. No./Substance:
0/Receptors, Nicotinic; 0/alpha7 nicotinic acetylcholine receptor; 0/nicotinic receptor beta2

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