| Distribution of newly synthesized lipoprotein over the outer membrane and the peptidoglycan sacculus of an Escherichia coli lac-lpp strain. | |
| | |
MedLine Citation:
|
PMID: 3316185 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The insertion of newly synthesized lipoprotein molecules into the cell wall of Escherichia coli was studied topographically by immunoelectron microscopy. Lipoprotein was briefly induced with isopropyl-beta-D-thiogalactopyranoside in cells carrying lac-lpp on a low-copy-number plasmid in an E. coli lpp host. Specific antibodies bound to the newly inserted lipoprotein molecules, which were exposed at the cell surface after treatment of the cells with Tris-EDTA, were detected with a protein A-gold probe. The average distribution of the gold particles over the cell surface of noninduced cells was determined for cells induced for 5 and 10 min. Analysis of 250 to 350 cells showed that the distribution of newly synthesized lipoprotein over the cell surface was homogeneous in both cases. The binding of lipoprotein to the peptidoglycan layer was studied by the same technique, and visual inspection again revealed a homogeneous distribution of bound lipoprotein over the entire sacculus surface. It is therefore concluded that free lipoprotein is inserted equally over the entire cell wall of E. coli, while binding to peptidoglycan also occurs over the entire cell surface. The rate of lipoprotein synthesis increased with cell length in nondividing cells, whereas it was constant in cells which had initiated constriction. Analysis of cells having different amounts of lipoprotein in their cell wall revealed that the cell shape depended on the total lipoprotein content of the cell. Cells having no or only a small amount of lipoprotein grew as spheres, whereas cells with increasing numbers of lipoprotein molecules gradually changed their shape to short rods. |
| | |
Authors:
|
H Hiemstra; N Nanninga; C L Woldringh; M Inouye; B Witholt |
Related Documents
:
|
1313035 - The effect of low density lipoproteins, cholesterol, and 25-hydroxycholesterol on apoli... 2156835 - Antigen receptor-mediated regulation of sustained polyphosphoinositide turnover in a hu... 8123685 - Fatty acid desaturation in an animal cell mutant defective in plasmanylethanolamine des... 18480495 - A mechanism accounting for the low cellular level of linoleic acid in cystic fibrosis a... 310825 - Antigen-binding t cells: dose response and kinetic studies on the development of differ... 7943285 - Nmr-visible intracellular p(i) and phosphoesters during regulation of na(+)-p(i) cotran... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Journal of bacteriology Volume: 169 ISSN: 0021-9193 ISO Abbreviation: J. Bacteriol. Publication Date: 1987 Dec |
Date Detail:
|
Created Date: 1988-01-14 Completed Date: 1988-01-14 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 2985120R Medline TA: J Bacteriol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 5434-44 Citation Subset: IM |
Affiliation:
|
Department of Biochemistry, University of Groningen, The Netherlands. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Bacterial Outer Membrane Proteins
/
analysis*,
biosynthesis Cell Wall / analysis Escherichia coli / analysis, genetics, growth & development, metabolism* Immunohistochemistry Lipoproteins / analysis*, biosynthesis Microscopy, Electron Peptidoglycan / analysis* Plasmids |
| Chemical | |
Reg. No./Substance:
|
0/Bacterial Outer Membrane Proteins; 0/Lipoproteins; 0/Peptidoglycan |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Nucleotide sequence of the iap gene, responsible for alkaline phosphatase isozyme conversion in Esch...
Next Document: Sulfur metabolism in Beggiatoa alba.