Document Detail


Distribution of nerve growth factor in cat brains following topical application of solution or Minipellet.
MedLine Citation:
PMID:  9522345     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The distribution of nerve growth factor (NGF) was studied after topical application of NGF solution or NGF Minipellet into the caudate nucleus of cat brains. Each aliquot of NGF solution or a piece of NGF Minipellet contains 200 micrograms of mouse 2.5S beta-NGF. The concentrations of NGF in various areas of the brain were determined by enzyme-linked immunosolvent assay (ELISA). The tissue concentrations of NGF were very high at 6 h after injection of NGF solution (124.0 +/- 2.46 to 2144.0 +/- 16.03 ng g-1), but quickly decreased on day 1. The tissue concentrations of NGF were low at 6 h after implantation of NGF Minipellet, but were considerably increased on day 1 (11.22 +/- 3.36 to 72.04 +/- 20.45 ng g-1), and slowly decreased during the following 6 to 14 days. The present study demonstrates the temporal and spatial profiles of NGF distribution in the brain of middle-sized animals after topical application. Injection of NGF solution resulted in abrupt, but very transient, elevation of tissue NGF. Implantation of NGF Minipellet maintained the tissue NGF at biologically effective levels for 6 to 14 days.
Authors:
O Takemoto; T Yoshimine; A K Muhammad; M Maruno; S Yamamoto; K Fujioka; N Koseki; Y Takada; A Sano; H Maeda; T Hayakawa
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neurological research     Volume:  20     ISSN:  0161-6412     ISO Abbreviation:  Neurol. Res.     Publication Date:  1998 Mar 
Date Detail:
Created Date:  1998-04-30     Completed Date:  1998-04-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7905298     Medline TA:  Neurol Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  116-20     Citation Subset:  IM    
Affiliation:
Department of Neurosurgery, Osaka University Medical School, Japan.
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MeSH Terms
Descriptor/Qualifier:
Administration, Topical
Animals
Brain / metabolism*
Cats / metabolism*
Drug Implants
Enzyme-Linked Immunosorbent Assay
Half-Life
Nerve Growth Factors / administration & dosage*,  pharmacokinetics*
Osmolar Concentration
Solutions
Time Factors
Tissue Distribution
Chemical
Reg. No./Substance:
0/Drug Implants; 0/Nerve Growth Factors; 0/Solutions

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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