Document Detail


Distribution and morphometric quantitation of pancreatic endocrine cell types in the frog, Rana pipiens.
MedLine Citation:
PMID:  6106438     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cells reactive to anti-anglerfish insulin, anti-porcine glucagon, anti-synthetic somatostatin, and anti-bovine pancreatic polypeptide were identified in adult Rana pipiens male pancreases using peroxidase anti-peroxidase immunohistochemistry. Insulin positive cells are columnar shaped and arranged in cords. Glucagon positive and somatostatin positive cells are located around the core of insulin positive cells. Isolated cells and clusters of cells of only one cell type are also found. Adjacent sections stained with anti-glucagon and anti-bovine pancreatic polypeptide showed that glucagon positivity and pancreatic polypeptide positivity are found in the same cells. Comparison of double stained adjacent sections confirmed the presence of these two antigens in the same cells, and further showed the occasional presence of cells which are positive to only glucagon or pancreatic polypeptide. Staining of rat pancreas with these two antisera showed that glucagon and pancreatic polypeptide are present in two distinct cell populations. Morphometric quantitation of immunohistochemically stained sections of Rana pipiens pancreases showed that about 2% of the pancreas is endocrine tissue. Of this, 43% is comprised of insulin positive cells, and 43% is occupied by glucagon-pancreatic polypeptide positive cells. Somatostatin positive cell occupy about 14% of the total islet volume. The presence of glucagon and pancreatic polypeptide in the same cell population in the frog, but in different cell populations in mammals, may reflect special functional adaptation in this species, or a close relation of these two hormones and their cells of production during evolution.
Authors:
H C Kaung; R P Elde
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Anatomical record     Volume:  196     ISSN:  0003-276X     ISO Abbreviation:  Anat. Rec.     Publication Date:  1980 Feb 
Date Detail:
Created Date:  1980-11-25     Completed Date:  1980-11-25     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370540     Medline TA:  Anat Rec     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  173-81     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Glucagon / immunology
Immunoenzyme Techniques
Insulin / immunology
Pancreas / cytology*,  immunology
Rana pipiens / anatomy & histology*
Somatostatin / immunology
Grant Support
ID/Acronym/Agency:
AM19868/AM/NIADDK NIH HHS
Chemical
Reg. No./Substance:
11061-68-0/Insulin; 51110-01-1/Somatostatin; 9007-92-5/Glucagon

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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