Document Detail

Distribution of monoclonal antibodies in intestinal and urogenital secretions of mice bearing hybridoma 'backpack' tumours.
MedLine Citation:
PMID:  9042427     Owner:  NLM     Status:  MEDLINE    
Mice bearing IgA hybridoma 'backpack' tumours have been used to demonstrate that secretion of a single monoclonal IgA can protect against mucosal infection, but the relevance of this model to normal IgA protection is not clear. The authors analysed the distribution of specific monoclonal and total antibodies in bile, local intestinal secretions, cervical-vaginal secretions, urine and serum of mice bearing anti-cholera toxin (CT) IgA and IgG backpack tumours, with and without bile duct ligation. Backpack tumours resulted in high levels of both anti-CT and total IgA or IgG in serum, and IgA (but not IgG) in bile. Secretions recovered by absorbent filter 'wicks' from mucosal surfaces throughout the intestines of backpack tumour mice contained significant concentrations of monoclonal anti-CT IgA, but total IgA levels were as in normal mice. Neither monoclonal nor total IgA levels on mucosal surfaces were altered by bile duct ligation. Furthermore, anti-CT monoclonal IgA levels in local intestinal secretions of backpack tumour mice were comparable to specific polyclonal IgA levels previously elicited by mucosal immunization with CT. Thus, IgA-mediated protection against enteric challenge in the backpack tumour model may be a valid predictor of protection provided by natural mucosal immunization in vivo. High monoclonal IgA levels in bile, urine and the female genital tract, however, may not reflect the situation in normal immunized mice.
B Haneberg; D Kendall; F M Apter; M R Neutra
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Scandinavian journal of immunology     Volume:  45     ISSN:  0300-9475     ISO Abbreviation:  Scand. J. Immunol.     Publication Date:  1997 Feb 
Date Detail:
Created Date:  1997-03-26     Completed Date:  1997-03-26     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0323767     Medline TA:  Scand J Immunol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  151-9     Citation Subset:  IM    
Department of Pediatrics, Harvard Medical School, Boston, MA, USA.
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MeSH Terms
Antibodies, Bacterial / blood,  immunology,  urine
Antibodies, Monoclonal / blood,  pharmacokinetics*,  urine
Antitoxins / analysis
Bile / immunology
Cervix Uteri / immunology
Cholera Toxin / immunology
Feces / chemistry
Hybridomas / immunology*
Immunoglobulin A / immunology*
Immunoglobulin G / immunology
Intestines / immunology*
Mice, Inbred BALB C
Neoplasms, Experimental / immunology
Urogenital System / immunology*
Vagina / immunology
Grant Support
Reg. No./Substance:
0/Antibodies, Bacterial; 0/Antibodies, Monoclonal; 0/Antitoxins; 0/Immunoglobulin A; 0/Immunoglobulin G; 9012-63-9/Cholera Toxin

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