Document Detail

Distribution and linkage of repetitive clusters from the heterochromatic region of human chromosome 22.
MedLine Citation:
PMID:  8817068     Owner:  NLM     Status:  MEDLINE    
The pericentric regions of eukaryotic chromosomes consist of several types of repetitive DNA families. In human chromosome 22, the organization of such families was studied in more detail. In addition to the known families of alpha and beta repeats, an additional repeat with a 48-bp motif was previously assigned to 22pter-q11. Here, we report in more detail the distribution of these repeat families, applying pulsed-field gel electrophoresis, fluorescence in situ hybridization and physical linkage on cosmid recombinants. At least two clusters of 48-bp repeats are localized on chromosome 22, one on the distal p-arm and one in the region 22cen-q11. Cosmids from a chromosome 22 library, containing both 48-bp and beta-repeats, link both arrays on 22p and define their maximum distances to less than 44 kb. Loss of 48-bp repeat sequences in a Dl-George cell line carrying a deletion in 22q11 suggests the presence of a second cluster in 22q11, a distribution supported by (fluorescene in situ hybridization)-FISH signal analysis. As additional members of the 48-bp repeat family can be found on all acrocentric chromosomes. It remains to be determined whether the distribution seen on chromosome 22 is also common in other human acrocentric chromosomes.
R Müllenbach; C Pusch; K Holzmann; R Suijkerbuijk; N Blin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology     Volume:  4     ISSN:  0967-3849     ISO Abbreviation:  Chromosome Res.     Publication Date:  1996 Jun 
Date Detail:
Created Date:  1996-11-27     Completed Date:  1996-11-27     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9313452     Medline TA:  Chromosome Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  282-7     Citation Subset:  IM    
ICRF Molecular Oncology Unit, Institute of Child Health, London, UK.
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MeSH Terms
Chromosome Mapping
Chromosomes, Human, Pair 22 / genetics*
Cosmids / genetics
Electrophoresis, Gel, Pulsed-Field
Heterochromatin / genetics*
In Situ Hybridization, Fluorescence
Linkage (Genetics)
Repetitive Sequences, Nucleic Acid / genetics*
Reg. No./Substance:

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