Document Detail

Distribution and induction of cytochrome P-450 and two cytochrome P-450-dependent monooxygenase activities in rat liver parenchymal cell subpopulations separated by centrifugal elutriation.
MedLine Citation:
PMID:  2742497     Owner:  NLM     Status:  MEDLINE    
Liver parenchymal cells from the periportal and centrilobular zones differ in their morphological, biochemical and functional characteristics. In an effort to obtain fractions enriched in either periportal or centrilobular cells, isolated rat liver parenchymal cells were separated into five subpopulations by centrifugal elutriation. The mean diameters of the cells present in fractions I-V were 19.6, 21.1, 21.8, 22.7 and 23.5 micron, respectively. The content of cytochrome P-450 as well as benzphetamine N-demethylase and 7-ethoxyresorufin O-deethylase activities were higher in the larger parenchymal cells than in the smaller ones. After administration of phenobarbital the content of cytochrome P-450 was approximately two-fold greater in the cells present in fractions 3-5, when compared to the same subpopulations isolated from untreated rats; the activity of benzphetamine N-demethylase was enhanced to a similar extent in all five fractions. 3-Methylcholanthrene treatment resulted in a significant increase of cytochrome P-450 content and 7-ethoxyresorufin O-deethylase activity in all five fractions: both parameters were slightly higher in fractions 4 and 5 than in fractions 1 and 2. In conclusion, the elutriated liver parenchymal cells seem to preserve the biochemical heterogeneity observed in the intact liver; the potential enrichment of periportal and centrilobular cells in the different fractions by centrifugal elutriation is discussed.
B Seibert; F Oesch; P Steinberg
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Archives of toxicology     Volume:  63     ISSN:  0340-5761     ISO Abbreviation:  Arch. Toxicol.     Publication Date:  1989  
Date Detail:
Created Date:  1989-08-10     Completed Date:  1989-08-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0417615     Medline TA:  Arch Toxicol     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  18-22     Citation Subset:  IM    
Institute of Toxicology, University of Mainz, Federal Republic of Germany.
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MeSH Terms
Alanine Transaminase / metabolism
Cytochrome P-450 CYP1A1
Cytochrome P-450 Enzyme System / biosynthesis,  metabolism*
Enzyme Induction / drug effects
Liver / cytology,  enzymology*
Methylcholanthrene / pharmacology
Mixed Function Oxygenases / biosynthesis,  metabolism*
Oxidoreductases / metabolism
Oxidoreductases, N-Demethylating / metabolism
Phenobarbital / pharmacology
Pyruvate Kinase / metabolism
Rats, Inbred Strains
Reg. No./Substance:
50-06-6/Phenobarbital; 56-49-5/Methylcholanthrene; 9035-51-2/Cytochrome P-450 Enzyme System; EC 1.-/Mixed Function Oxygenases; EC 1.-/Oxidoreductases; EC P-450 CYP1A1; EC N-demethylase; EC 1.5.-/Oxidoreductases, N-Demethylating; EC Transaminase; EC Kinase

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