Document Detail

Distribution of individual components of basement membrane in human colon polyps and adenocarcinomas as revealed by monoclonal antibodies.
MedLine Citation:
PMID:  1371500     Owner:  NLM     Status:  MEDLINE    
Double-label immunofluorescence was used to monitor basement-membrane composition and integrity in 22 human colon polyps, 36 adenocarcinomas and 2 metastases. Cryostat sections were stained with polyclonal anti-laminin anti-serum combined with monoclonal antibodies (MAbs) to all major basement-membrane components (laminin, entactin/nidogen, collagen type IV and large heparan sulfate proteoglycan), as well as to keratin 8. In all adenocarcinomas, including mucinous, basement membranes were altered more at the invasive front than in the parenchyma. The degree of this alteration was inversely correlated with the level of tumor differentiation. An uncoordinated loss of basement membrane components (dissociation of markers), previously described by us in rat colon adenocarcinomas, was also found in human tumors. In the great majority of adenocarcinomas a pronounced stromal reaction was seen. It was manifested by the presence of fibrillar deposits of basement-membrane components, mainly of collagen type IV and/or heparan sulfate proteoglycan. This reaction was never observed in polyps and may be derived from myofibroblasts reported to accumulate in colon cancer stroma. The combined use of antibodies to basement-membrane components and to a specific keratin may constitute an adequate immunohistochemical test for the presence of invasion, and may be useful in the histologic analysis of polyps, especially in dubious cases.
A V Ljubimov; J Bartek; J R Couchman; L L Kapuller; V V Veselov; J Kovarik; A G Perevoshchikov; V A Krutovskikh
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  International journal of cancer. Journal international du cancer     Volume:  50     ISSN:  0020-7136     ISO Abbreviation:  Int. J. Cancer     Publication Date:  1992 Feb 
Date Detail:
Created Date:  1992-03-30     Completed Date:  1992-03-30     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0042124     Medline TA:  Int J Cancer     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  562-6     Citation Subset:  IM    
All-Union Cancer Research Center, USSR AMS, Moscow.
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MeSH Terms
Adenocarcinoma / metabolism*
Antibodies, Monoclonal
Basement Membrane / metabolism*
Blotting, Western
Collagen / metabolism
Colon / metabolism*
Extracellular Matrix Proteins / metabolism*
Fluorescent Antibody Technique
Heparan Sulfate Proteoglycans
Heparitin Sulfate / metabolism
Intestinal Neoplasms / metabolism*
Intestinal Polyps / metabolism*
Keratins / metabolism
Laminin / metabolism
Lymphatic Metastasis
Membrane Glycoproteins / metabolism
Proteoglycans / metabolism
Grant Support
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Extracellular Matrix Proteins; 0/Heparan Sulfate Proteoglycans; 0/Laminin; 0/Membrane Glycoproteins; 0/Proteoglycans; 0/nidogen; 68238-35-7/Keratins; 9007-34-5/Collagen; 9050-30-0/Heparitin Sulfate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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