| Distribution and in-vitro transfer of tetracycline resistance determinants in clinical and aquatic Acinetobacter strains. | |
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MedLine Citation:
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PMID: 11023190 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Following characterisation by phenotypic tests and amplified ribosomal DNA restriction analysis (ARDRA), 50 tetracycline-resistant (MIC > or = 16 mg/L) Acinetobacter strains from clinical (n = 35) and aquatic (n = 15) samples were analysed by PCR for tetracycline resistance (Tet) determinants of classes A-E. All the clinical strains were A. baumannii; most (33 of 35) had Tet A (n = 16) or B (n = 17) determinants, and only two did not yield amplicons with primers for any of the five tetracycline resistance determinants. The aquatic strains belonged to genomic species other than A. baumannii, and most (12 of 15) did not contain determinants Tet A-E. Strains negative for Tet A-E were also negative for Tet G and M; further analysis of two aquatic strains with specific primers for Tet O and Tet Y and degenerate primers for Tet M-S-O-P(B)-Q also showed negative results. Transfer of tetracycline resistance was tested for 20 strains with three aquatic Acinetobacter strains and Escherichia coli K-12 as recipients. Transfer of resistance was demonstrated between aquatic strains from distinct ecological niches, but not from clinical to aquatic strains, nor from any Acinetobacter strain to E. coli K-12. Most transconjugants acquired multiple relatively small plasmids (<36 kb). Transfer did not occur when DNA from the donor strains was added to the recipient cultures and was not affected by deoxyribonuclease I, suggesting a conjugative mechanism. It is concluded that Tet A and B are widespread among tetracycline-resistant A. baumannii strains of clinical origin, but unknown genetic determinants are responsible for most tetracycline resistance among aquatic Acinetobacter spp. These differences, together with the inability of clinical strains to transfer tetracycline resistance in vitro to aquatic strains, contra-indicate any important flow of tetracycline resistance genes between clinical and aquatic acinetobacter populations. |
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Authors:
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L Guardabassi; L Dijkshoorn; J M Collard; J E Olsen; A Dalsgaard |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of medical microbiology Volume: 49 ISSN: 0022-2615 ISO Abbreviation: J. Med. Microbiol. Publication Date: 2000 Oct |
Date Detail:
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Created Date: 2000-10-19 Completed Date: 2000-10-19 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0224131 Medline TA: J Med Microbiol Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 929-36 Citation Subset: IM |
Affiliation:
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Department of Veterinary Microbiology, The Royal Veterinary and Agricultural University, Frederiksberg, Denmark. lg@kvl.dk |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acinetobacter
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classification,
drug effects*,
genetics Acinetobacter Infections / drug therapy*, microbiology Anti-Bacterial Agents / pharmacology*, therapeutic use DNA Primers / chemistry DNA Restriction Enzymes / chemistry DNA, Ribosomal / chemistry Drug Resistance, Microbial Electrophoresis, Agar Gel Gene Transfer Techniques Humans Plasmids / chemistry Polymerase Chain Reaction Tetracycline / pharmacology*, therapeutic use Tetracycline Resistance / genetics* Water Microbiology* |
| Chemical | |
Reg. No./Substance:
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0/Anti-Bacterial Agents; 0/DNA Primers; 0/DNA, Ribosomal; 60-54-8/Tetracycline; EC 3.1.21.-/DNA Restriction Enzymes |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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