| Distribution of immunogenic cells after painting with the contact sensitizers fluorescein isothiocyanate and oxazolone. Different sensitizers form immunogenic complexes with different cell populations. | |
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MedLine Citation:
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PMID: 6991394 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The distribution of fluorescent cells in the draining lymph nodes of mice painted with the contact sensitizing agent fluorescein isothiocyanate (FITC) was investigated using a fluorescence-activated cell sorter. Up to 30% of the cells were fluorescent after 18 h and this decreased thereafter becoming undetectable after 4-5 days. Most of the fluorescent cells were morphologically lymphocytes, theta - ve and adherent to nylon wool. Immunogenicity of these cells was tested by injecting them into the footpads of normal mice and measuring contact sensitivity after 6 days. This was restricted to large cells which represented less than 5% of the white cell population and nearly all of which became fluorescent after skin painting. The large fluorescent cells were a mixture of monocytes and lymphocytes. Most of the lymphocytes had surface immunoglobulin. The immunogenicity was reduced by nylon filtration but was not affected by silica and anti-theta. These results showed that the immunogenicity is not associated with T cells. In contrast, similar immunogenic activity in the draining lymph nodes of mice painted with oxazolone is associated with T cells. The results therefore showed that different sensitizers form immunogenic complexes with different cell populations, perhaps in this case becuase of the different water solubilities of FITC and oxazolone. They also suggested that this may cause important differences in antigen presentation, for example in their association with different MHC products. |
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Authors:
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W R Thomas; A J Edwards; M C Watkins; G L Asherson |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Immunology Volume: 39 ISSN: 0019-2805 ISO Abbreviation: Immunology Publication Date: 1980 Jan |
Date Detail:
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Created Date: 1980-08-28 Completed Date: 1980-08-28 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0374672 Medline TA: Immunology Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 21-7 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibody-Producing Cells* Cell Separation Dermatitis, Contact / immunology* Fluoresceins Fluorescent Antibody Technique Lymph Nodes / immunology* Lymphocytes / immunology Male Mice Mice, Inbred CBA Mice, Nude Monocytes / immunology Oxazolone Receptors, Antigen, B-Cell |
| Chemical | |
Reg. No./Substance:
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0/Fluoresceins; 0/Receptors, Antigen, B-Cell; 15646-46-5/Oxazolone |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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