Document Detail


Distribution of extracellular matrix proteins type I collagen, type IV collagen, fibronectin, and laminin in mouse folliculogenesis.
MedLine Citation:
PMID:  16758163     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The extracellular matrix (ECM) plays a prominent role in ovarian function by participating in processes such as cell migration, proliferation, growth, and development. Although some of these signaling processes have been characterized in the mouse, the relative quantity and distribution of ECM proteins within developing follicles of the ovary have not been characterized. This study uses immunohistochemistry and real-time PCR to characterize the ECM components type I collagen, type IV collagen, fibronectin, and laminin in the mouse ovary according to follicle stage and cellular compartment. Collagen I was present throughout the ovary, with higher concentrations in the ovarian surface epithelium and follicular compartments. Collagen IV was abundant in the theca cell compartment with low-level expression in the stroma and granulosa cells. The distribution of collagen was consistent throughout follicle maturation. Fibronectin staining in the stroma and theca cell compartment increased throughout follicle development, while staining in the granulosa cell compartment decreased. Heavy staining was also observed in the follicular fluid of antral follicles. Laminin was localized primarily to the theca cell compartment, with a defined ring at the exterior of the follicular granulosa cells marking the basement membrane. Low levels of laminin were also apparent in the stroma and granulosa cell compartment. Taken together, the ECM content of the mouse ovary changes during follicular development and reveals a distinct spatial and temporal pattern. This understanding of ECM composition and distribution can be used in the basic studies of ECM function during follicle development, and could aid in the development of in vitro systems for follicle growth.
Authors:
Courtney B Berkholtz; Bonnie E Lai; Teresa K Woodruff; Lonnie D Shea
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2006-06-07
Journal Detail:
Title:  Histochemistry and cell biology     Volume:  126     ISSN:  0948-6143     ISO Abbreviation:  Histochem. Cell Biol.     Publication Date:  2006 Nov 
Date Detail:
Created Date:  2006-10-03     Completed Date:  2007-04-25     Revised Date:  2014-09-24    
Medline Journal Info:
Nlm Unique ID:  9506663     Medline TA:  Histochem Cell Biol     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  583-92     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Collagen Type I / metabolism*
Collagen Type IV / metabolism*
Female
Fibronectins / metabolism*
Granulosa Cells / metabolism
Immunohistochemistry
Laminin / metabolism*
Male
Mice
Mice, Inbred C57BL
Ovarian Follicle / growth & development*
Ovary / metabolism*
RNA, Messenger / metabolism
Stromal Cells / metabolism
Theca Cells / metabolism
Grant Support
ID/Acronym/Agency:
U54 HD041857/HD/NICHD NIH HHS; U54 HD041857/HD/NICHD NIH HHS; U54 HD041857-01A1/HD/NICHD NIH HHS; U54 HD041857-01A10001/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Collagen Type I; 0/Collagen Type IV; 0/Fibronectins; 0/Laminin; 0/RNA, Messenger
Comments/Corrections

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