Document Detail


Distribution and diversity of the enterococcal surface protein (esp) gene in animal hosts and the Pacific coast environment.
MedLine Citation:
PMID:  19187132     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
AIMS: This study sought to evaluate the distribution of the enterococcal surface protein (esp) gene in Enterococcus faecium in the Pacific coast environment as well as the distribution and diversity of the gene in Northern California animal hosts. METHODS AND RESULTS: Over 150 environmental samples from the Pacific coast environment (sand, surf zone, fresh/estuarine, groundwater, and storm drain) were screened for the esp gene marker in E. faecium, and the marker was found in 37% of the environmental samples. We examined the host specificity of the gene by screening various avian and mammalian faecal samples, and found the esp gene to be widespread in nonhuman animal faeces. DNA sequence analysis performed on esp polymerase chain reaction amplicons revealed that esp gene sequences were not divergent between hosts. CONCLUSIONS: Our data confirm recent findings that the E. faecium variant of the esp gene is not human-specific. SIGNIFICANCE AND IMPACT OF THE STUDY: Our results suggest that the use of the esp gene for microbial source tracking applications may not be appropriate at all recreational beaches.
Authors:
B A Layton; S P Walters; A B Boehm
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2009-02-02
Journal Detail:
Title:  Journal of applied microbiology     Volume:  106     ISSN:  1365-2672     ISO Abbreviation:  J. Appl. Microbiol.     Publication Date:  2009 May 
Date Detail:
Created Date:  2009-04-28     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9706280     Medline TA:  J Appl Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1521-31     Citation Subset:  IM    
Affiliation:
Environmental and Water Studies, Department of Civil and Environmental Engineering, Stanford University, Stanford, CA 94305, USA.
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