| Distribution and dietary induction of cellular retinol-binding protein type II along the villus-crypt axis of the rat jejunum. | |
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MedLine Citation:
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PMID: 18490842 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cellular retinol-binding protein type II (CRBPII) is exclusively expressed in the small intestinal absorptive cells. We previously reported that dietary fat induces CRBPII expression within 12 h of fat intake. To examine at which locus of the villus-crypt axis this response to dietary fat occurs, 6-wk-old rats were fed a low-fat diet (7% energy) for 7 d, and then given free access to a high-fat diet (70% energy) for the subsequent 12, 24 or 48 h. Cryostat sectioning of jejunal segments followed by RNA blot hybridization of the transcripts revealed that CRBPII mRNA was expressed maximally in the lower villus, and the immunoreactive protein of CRBPII was expressed maximally in the mid-villus. Feeding the high-fat diet caused a pronounced increase in CRBPII mRNA level from the lower- to middle-villus within 12 h. These results suggest that the CRBPII gene is maximally expressed in the lower villus, and that dietary fat causes an enhancement of CRBPII gene expression in the villus cells. |
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Authors:
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Yuko Ogura; Hiromitsu Yasutake; Kazuki Mochizuki; Saori Yoshikawa; Kazuhito Suruga; Hiroshi Sugiyama; Sachiko Takase; Toshinao Goda |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of nutritional science and vitaminology Volume: 54 ISSN: 0301-4800 ISO Abbreviation: J. Nutr. Sci. Vitaminol. Publication Date: 2008 Apr |
Date Detail:
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Created Date: 2008-05-20 Completed Date: 2008-09-23 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0402640 Medline TA: J Nutr Sci Vitaminol (Tokyo) Country: Japan |
Other Details:
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Languages: eng Pagination: 130-5 Citation Subset: IM |
Affiliation:
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Laboratory of Nutritional Physiology and the Global COE Program, School of Food and Nutritional Sciences, The University of Shizuoka, Shizuoka, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blotting, Northern / methods Diet / methods Dietary Fats / administration & dosage, pharmacology* Intestinal Mucosa / cytology, drug effects, metabolism Jejunum / cytology, drug effects, metabolism* Male Microvilli / drug effects, metabolism Rats Rats, Sprague-Dawley Retinol-Binding Proteins, Cellular / metabolism* Tissue Distribution |
| Chemical | |
Reg. No./Substance:
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0/Dietary Fats; 0/Retinol-Binding Proteins, Cellular |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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