Document Detail

Distribution of corticotropin-releasing factor in ovine brain determined by radioimmunoassay.
MedLine Citation:
PMID:  6600847     Owner:  NLM     Status:  MEDLINE    
We have developed and used a sensitive and specific radioimmunoassay to demonstrate the presence of CRF-like immunoreactivity in extra-hypothalamic areas of ovine brain. Synthetic CRF displaced antibody bound tracer at an ED50 value of 200 pg and there was no cross-reactivity with LHRH, TRH, ACTH, beta-endorphin and several other peptides. Displacement of bound 125I-CRF by brain extracts exhibited curves parallel to synthetic CRF standards. Highest concentrations (1 ng/mg tissue) of CRF-like immunoreactivity were found in the median eminence but surprisingly, high concentrations of CRF-like immunoreactivity were found in frontal, parietal, occipital and particularly temporal areas of cerebral cortex. Much lower concentrations were found in other brain areas including the basal ganglia, limbic system and brain stem.
J Côté; G Lefèvre; F Labrie; N Barden
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Regulatory peptides     Volume:  5     ISSN:  0167-0115     ISO Abbreviation:  Regul. Pept.     Publication Date:  1983 Jan 
Date Detail:
Created Date:  1983-04-15     Completed Date:  1983-04-15     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  8100479     Medline TA:  Regul Pept     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  189-95     Citation Subset:  IM    
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MeSH Terms
Brain / metabolism*
Brain Mapping
Corticotropin-Releasing Hormone / immunology,  metabolism*
Tissue Distribution
Reg. No./Substance:
9015-71-8/Corticotropin-Releasing Hormone

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