Document Detail

Distribution of cells labelled by a monoclonal antibody (A3) against a cloned cell line derived from a rat malignant fibrous histiocytoma.
MedLine Citation:
PMID:  11032659     Owner:  NLM     Status:  MEDLINE    
To pursue the histogenesis of malignant fibrous histiocytoma (MFH), of which the cell of origin is still debated, a monoclonal antibody (A3) was produced against a rat MFH-derived cloned cell line (MT-8). Antigen recognized by A3 was around 80 kDa in molecular weight and was seen on the cytoplasmic membrane of MT-8 cells by immunoelectron microscopy. A3 reacted specifically with MT-8 cells, with another rat MFH-derived cell line (MT-9) and with their induced tumours in syngeneic rats, but not with other rat tumours such as fibrosarcoma, histiocytic sarcoma, malignant meningioma, uterine leiomyosarcoma, endometrial stromal sarcoma, mononuclear cell leukaemia and malignant schwannoma. These findings indicate that A3 has a high specificity for rat MFH cells. In fetuses on gestation days 15, 18 and 20 and in postnatal rats aged 1, 4 and 8 days, A3 reacted with primitive mesenchymal cells in visceral organs and around arteries and bronchi, as well as in the lamina propria of intestinal mucosa, renal interstitium, meninges and perineurium. There were no A3-positive connective tissue cells in organs or other sites in adult rats more than 10 weeks old. It is therefore likely that MFH cells share antigens with primitive mesenchymal cells, which may be multipotent for mesenchymal differentiation. The present study suggests that MFH consists of a population of primitive, undifferentiated mesenchymal cells. A3 also immunolabelled endothelial cells of arteries, venules and pulmonary capillaries in fetal, postnatal and adult rats; vascular endothelial cells in chemically induced hepatic and renal lesions also reacted strongly with A3. However, the significance of endothelial immunoreactivity with A3 remains to be elucidated.
D Kumagai; J Yamate; T Tajima; Y Tsukamoto; H Yasui; M Kuwamura; T Kotani; S Sakuma
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of comparative pathology     Volume:  123     ISSN:  0021-9975     ISO Abbreviation:  J. Comp. Pathol.     Publication Date:    2000 Aug-Oct
Date Detail:
Created Date:  2000-11-03     Completed Date:  2000-11-21     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0102444     Medline TA:  J Comp Pathol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  77-87     Citation Subset:  IM    
Copyright Information:
Copyright 2000 Harcourt Publishers Ltd.
Veterinary Teaching Hospital, College of Agriculture, Osaka Prefecture University, Gakuencho 1-1, Sakai, Osaka, 599-8531, Japan.
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MeSH Terms
Antibodies, Monoclonal / analysis,  pharmacokinetics*
Aorta / chemistry,  embryology
Carbon Tetrachloride / adverse effects
Cisplatin / adverse effects
Drug-Induced Liver Injury
Histiocytoma, Benign Fibrous / immunology,  metabolism*,  pathology
Kidney Diseases / chemically induced,  metabolism,  pathology
Liver / chemistry,  embryology
Liver Diseases / metabolism,  pathology
Lung / chemistry,  embryology,  ultrastructure
Mice, Inbred BALB C
Microscopy, Immunoelectron
Rats, Inbred F344
Spinal Cord / chemistry,  embryology
Tumor Cells, Cultured / immunology,  metabolism,  ultrastructure
Reg. No./Substance:
0/Antibodies, Monoclonal; 15663-27-1/Cisplatin; 56-23-5/Carbon Tetrachloride

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