| Distribution of cardiac myosin isozymes in human conduction system. Immunohistochemical study using monoclonal antibodies. | |
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MedLine Citation:
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PMID: 3511096 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To determine the presence and distribution of cardiac myosin isozymes in the human conduction system, we performed an immunohistochemical study using monoclonal antibodies CMA19 and HMC14, which are specific for myosin heavy chains of human atrial type (alpha-type) and ventricular type (beta-type), respectively. Serial frozen sections of human hearts were obtained from autopsy samples and examined by indirect immunofluorescence. Alpha-type was found in all myofibers of sinus node and atrio-ventricular node, and in 55.2 +/- 10.2% (mean +/- SD, n = 5) of the myofibers of ventricular conduction tissue, which consists of the bundle of His, bundle branches, and the Purkinje network. In contrast, beta-type was found in all myofibers of the atrio-ventricular node and ventricular conduction tissue, whereas almost all myofibers of the sinus node were unlabeled by HMC14. Although the number of ventricular myofibers labeled by CMA19 was small, the labeled myofibers were more numerous in the subepicardial region than in the subendocardial region. These findings show that the gene coding for alpha-type is expressed predominantly in specialized myocardium compared with the adjacent ordinary working myocardium. |
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Authors:
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M Kuro-o; H Tsuchimochi; S Ueda; F Takaku; Y Yazaki |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of clinical investigation Volume: 77 ISSN: 0021-9738 ISO Abbreviation: J. Clin. Invest. Publication Date: 1986 Feb |
Date Detail:
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Created Date: 1986-03-26 Completed Date: 1986-03-26 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 7802877 Medline TA: J Clin Invest Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 340-7 Citation Subset: AIM; IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antibodies, Monoclonal Atrioventricular Node / analysis Bundle of His / analysis Fluorescent Antibody Technique Heart Atria / analysis Heart Conduction System / analysis* Heart Ventricles / analysis Histocytochemistry Humans Myosins / analysis* Purkinje Fibers / analysis Sinoatrial Node / analysis Tissue Distribution |
| Chemical | |
Reg. No./Substance:
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0/Antibodies, Monoclonal; EC 3.6.4.1/Myosins |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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