Document Detail

Distribution of calbindin-D28k, neuronal nitric oxide synthase, and nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d) in the lateral nucleus of the sheep amygdaloid complex.
MedLine Citation:
PMID:  17047987     Owner:  NLM     Status:  MEDLINE    
This study describes calbindin-D28k (CB), neuronal nitric oxide synthase (nNOS), and nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d) expression in the lateral nucleus of the sheep amygdaloid complex. Double immunofluorescence protocol was used in order to determine whether there is colocalization of CB and nNOS. The CB-immunoreactive (IR) neuronal population was composed especially of non-pyramidal neurons, but a few pyramidal cells were also present. The non-pyramidal neurons showed a multipolar and, occasionally, a fusiform morphology. The comparison between single-labeled CB-IR non-pyramidal neurons and cells belonging to CB-IR neuronal population showed they were identical for morphology, mean size, and distribution. The single-labeled CB-IR non-pyramidal neurons were only the 17.8% of the total non-pyramidal neurons counted. The nNOS-IR neuronal population was represented by non-pyramidal multipolar and fusiform neurons. Single-labeled nNOS-IR non-pyramidal neurons had the same morphology, mean area, and distribution as cells belonging to nNOS-IR neuronal population. Single-labeled nNOS-IR non-pyramidal neurons were more numerous than single-labeled CB-IR, and represented the 73.7% of total non-pyramidal neurons counted. NADPH-d-positive cells had the same morphology and distribution as the nNOS-IR neurons. Double immunolabeling (CB/nNOS) was found mostly in non-pyramidal multipolar neurons and only in a few non-pyramidal fusiform cells. These neurons had a mean perikaryal area significantly higher and significantly smaller than that of single-labeled nNOS and single-labeled CB-IR non-pyramidal neurons, respectively. CB and nNOS coexist only in a minority of non-pyramidal neurons (8.5%). The 32.4% of all CB-IR non-pyramidal neurons were nNOS-positive; only 10.4% of nNOS-IR non-pyramidal neurons were CB-positive. These results indicate that CB and nNOS are expressed by selective neurons and that the majority of nNOS-IR non-pyramidal neurons are lacking in CB.
Cristiano Bombardi; Annamaria Grandis; Roberto Chiocchetti; Maria Luisa Lucchi
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Publication Detail:
Type:  Journal Article     Date:  2006-10-18
Journal Detail:
Title:  Anatomy and embryology     Volume:  211     ISSN:  0340-2061     ISO Abbreviation:  Anat. Embryol.     Publication Date:  2006 Nov 
Date Detail:
Created Date:  2006-11-30     Completed Date:  2007-03-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7505194     Medline TA:  Anat Embryol (Berl)     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  707-20     Citation Subset:  IM    
Department of Veterinary Morphophysiology and Animal Productions, Faculty of Veterinary Medicine, University of Bologna, Via Tolara di Sopra, 50, 40064, Ozzano dell'Emilia (BO), Italy.
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MeSH Terms
Amygdala / cytology,  metabolism*
Calcium-Binding Protein, Vitamin D-Dependent / metabolism*
Models, Biological
NADPH Dehydrogenase / metabolism*
Neurons / metabolism
Neuropil / metabolism
Nitric Oxide Synthase Type I / metabolism*
Sheep / metabolism*
Temporal Lobe / metabolism
Tissue Distribution
Reg. No./Substance:
0/Calcium-Binding Protein, Vitamin D-Dependent; 0/calbindin; EC Oxide Synthase Type I; EC Dehydrogenase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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