| Distribution of calbindin-D28k, neuronal nitric oxide synthase, and nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d) in the lateral nucleus of the sheep amygdaloid complex. | |
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MedLine Citation:
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PMID: 17047987 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This study describes calbindin-D28k (CB), neuronal nitric oxide synthase (nNOS), and nicotinamide adenine dinucleotide phosphate diaphorase (NADPH-d) expression in the lateral nucleus of the sheep amygdaloid complex. Double immunofluorescence protocol was used in order to determine whether there is colocalization of CB and nNOS. The CB-immunoreactive (IR) neuronal population was composed especially of non-pyramidal neurons, but a few pyramidal cells were also present. The non-pyramidal neurons showed a multipolar and, occasionally, a fusiform morphology. The comparison between single-labeled CB-IR non-pyramidal neurons and cells belonging to CB-IR neuronal population showed they were identical for morphology, mean size, and distribution. The single-labeled CB-IR non-pyramidal neurons were only the 17.8% of the total non-pyramidal neurons counted. The nNOS-IR neuronal population was represented by non-pyramidal multipolar and fusiform neurons. Single-labeled nNOS-IR non-pyramidal neurons had the same morphology, mean area, and distribution as cells belonging to nNOS-IR neuronal population. Single-labeled nNOS-IR non-pyramidal neurons were more numerous than single-labeled CB-IR, and represented the 73.7% of total non-pyramidal neurons counted. NADPH-d-positive cells had the same morphology and distribution as the nNOS-IR neurons. Double immunolabeling (CB/nNOS) was found mostly in non-pyramidal multipolar neurons and only in a few non-pyramidal fusiform cells. These neurons had a mean perikaryal area significantly higher and significantly smaller than that of single-labeled nNOS and single-labeled CB-IR non-pyramidal neurons, respectively. CB and nNOS coexist only in a minority of non-pyramidal neurons (8.5%). The 32.4% of all CB-IR non-pyramidal neurons were nNOS-positive; only 10.4% of nNOS-IR non-pyramidal neurons were CB-positive. These results indicate that CB and nNOS are expressed by selective neurons and that the majority of nNOS-IR non-pyramidal neurons are lacking in CB. |
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Authors:
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Cristiano Bombardi; Annamaria Grandis; Roberto Chiocchetti; Maria Luisa Lucchi |
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Publication Detail:
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Type: Journal Article Date: 2006-10-18 |
Journal Detail:
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Title: Anatomy and embryology Volume: 211 ISSN: 0340-2061 ISO Abbreviation: Anat. Embryol. Publication Date: 2006 Nov |
Date Detail:
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Created Date: 2006-11-30 Completed Date: 2007-03-01 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7505194 Medline TA: Anat Embryol (Berl) Country: Germany |
Other Details:
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Languages: eng Pagination: 707-20 Citation Subset: IM |
Affiliation:
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Department of Veterinary Morphophysiology and Animal Productions, Faculty of Veterinary Medicine, University of Bologna, Via Tolara di Sopra, 50, 40064, Ozzano dell'Emilia (BO), Italy. cbombardi@vet.unibo.it |
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| MeSH Terms | |
Descriptor/Qualifier:
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Amygdala
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cytology,
metabolism* Animals Calcium-Binding Protein, Vitamin D-Dependent / metabolism* Female Immunohistochemistry Models, Biological NADPH Dehydrogenase / metabolism* Neurons / metabolism Neuropil / metabolism Nitric Oxide Synthase Type I / metabolism* Sheep / metabolism* Temporal Lobe / metabolism Tissue Distribution |
| Chemical | |
Reg. No./Substance:
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0/Calcium-Binding Protein, Vitamin D-Dependent; 0/calbindin; EC 1.14.13.39/Nitric Oxide Synthase Type I; EC 1.6.99.1/NADPH Dehydrogenase |
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