| Distribution of calbindin-D28K in the brain of the fetal sheep in late gestation. | |
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MedLine Citation:
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PMID: 7796143 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Immunocytochemical distribution of calbindin-D28K was examined in the brains of fetal sheep at 100-105 and 120-125 days of gestation (dGA) and at term in labor (term approximately 150 dGA) and compared to adult sheep. Brains were perfused, cut frozen and immunostained as freely floating sections with a polyclonal anti-calbindin-D28K antibody. Light microscopy revealed that calbindin-D28K immunogenicity in fetal cerebellum, rhombencephalon, mesencephalon, diencephalon and telencephalon was overall very similar to that seen in the brains of adult sheep. In addition even though fetal sheep peripheral plasma cortisol concentrations increase 10-20 times over the last 3-4 weeks of gestation to peak during labor and delivery, no glucocorticoid dependent increases in calbindin-D28K in the hippocampal formation could be seen in the sheep fetus as have been demonstrated for the adult rat given exogenous glucocorticoids. It is concluded that: (1) the basic pattern of calbindin-D28K distribution seen in adult sheep brains is established by at least 100 dGA in the sheep fetus; and (2) the pattern of calbindin-D28K expression in fetal sheep hippocampal formation is not related to peripheral plasma glucocorticoid concentrations. |
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Authors:
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T J McDonald; C Li; R H Wasserman |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Brain research Volume: 675 ISSN: 0006-8993 ISO Abbreviation: Brain Res. Publication Date: 1995 Mar |
Date Detail:
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Created Date: 1995-08-03 Completed Date: 1995-08-03 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0045503 Medline TA: Brain Res Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 303-15 Citation Subset: IM |
Affiliation:
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College of Veterinary Medicine, Department of Physiology, Cornell University, Ithaca, NY 14853, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Brain / cytology, embryology* Brain Chemistry / physiology* Calcium-Binding Protein, Vitamin D-Dependent / metabolism* Female Gestational Age Glucocorticoids / blood Immunohistochemistry Nerve Fibers / metabolism Nerve Tissue Proteins / metabolism* Neurons / metabolism Pregnancy Sheep Trigeminal Nerve / metabolism |
| Grant Support | |
ID/Acronym/Agency:
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DK04652/DK/NIDDK NIH HHS; HD 21350/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Calcium-Binding Protein, Vitamin D-Dependent; 0/Glucocorticoids; 0/Nerve Tissue Proteins; 0/calbindin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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