Document Detail


Distribution of calbindin-D28K in the brain of the fetal sheep in late gestation.
MedLine Citation:
PMID:  7796143     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Immunocytochemical distribution of calbindin-D28K was examined in the brains of fetal sheep at 100-105 and 120-125 days of gestation (dGA) and at term in labor (term approximately 150 dGA) and compared to adult sheep. Brains were perfused, cut frozen and immunostained as freely floating sections with a polyclonal anti-calbindin-D28K antibody. Light microscopy revealed that calbindin-D28K immunogenicity in fetal cerebellum, rhombencephalon, mesencephalon, diencephalon and telencephalon was overall very similar to that seen in the brains of adult sheep. In addition even though fetal sheep peripheral plasma cortisol concentrations increase 10-20 times over the last 3-4 weeks of gestation to peak during labor and delivery, no glucocorticoid dependent increases in calbindin-D28K in the hippocampal formation could be seen in the sheep fetus as have been demonstrated for the adult rat given exogenous glucocorticoids. It is concluded that: (1) the basic pattern of calbindin-D28K distribution seen in adult sheep brains is established by at least 100 dGA in the sheep fetus; and (2) the pattern of calbindin-D28K expression in fetal sheep hippocampal formation is not related to peripheral plasma glucocorticoid concentrations.
Authors:
T J McDonald; C Li; R H Wasserman
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Brain research     Volume:  675     ISSN:  0006-8993     ISO Abbreviation:  Brain Res.     Publication Date:  1995 Mar 
Date Detail:
Created Date:  1995-08-03     Completed Date:  1995-08-03     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0045503     Medline TA:  Brain Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  303-15     Citation Subset:  IM    
Affiliation:
College of Veterinary Medicine, Department of Physiology, Cornell University, Ithaca, NY 14853, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Brain / cytology,  embryology*
Brain Chemistry / physiology*
Calcium-Binding Protein, Vitamin D-Dependent / metabolism*
Female
Gestational Age
Glucocorticoids / blood
Immunohistochemistry
Nerve Fibers / metabolism
Nerve Tissue Proteins / metabolism*
Neurons / metabolism
Pregnancy
Sheep
Trigeminal Nerve / metabolism
Grant Support
ID/Acronym/Agency:
DK04652/DK/NIDDK NIH HHS; HD 21350/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/Calcium-Binding Protein, Vitamin D-Dependent; 0/Glucocorticoids; 0/Nerve Tissue Proteins; 0/calbindin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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