| Distribution of apolipoproteins A-I and A-IV among lipoprotein classes in rat mesenteric lymph, fractionated by molecular sieve chromatography. | |
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MedLine Citation:
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PMID: 3081040 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The distribution of apolipoproteins A-I and A-IV among lymph lipoprotein fractions was studied after separation by molecular sieve chromatography, avoiding any ultracentrifugation. Lymph was obtained from rats infused either with a glucose solution or with a triacylglycerol emulsion. Relative to glucose infusion, triacylglycerol infusion caused a 20-fold increase in the output of triacylglycerol, coupled with a 4-fold increase in output of apolipoprotein A-IV. The output of apolipoprotein A-I was only elevated 2-fold. Chromatography on 6% agarose showed that lymph apolipoproteins A-I and A-IV are present on triacylglycerol-rich particles and on particles of the size of HDL. In addition, apolipoprotein A-IV is also present as 'free' apolipoprotein A-IV. The increase in apolipoprotein A-I output is caused by a higher output of A-I associated with large chylomicrons only, while the increase in apolipoprotein A-IV output is reflected by an increased output in all lymph lipoprotein fractions, including lymph HDL and 'free' apolipoprotein A-IV. The increased level of 'free' A-IV, seen in fatty lymph, may contribute to, and at least partly explain, the high concentrations of 'free' apolipoprotein A-IV present in serum obtained from fed animals. |
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Authors:
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G M Dallinga-Thie; A van Tol; F M van't Hooft; P H Groot |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 876 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1986 Mar |
Date Detail:
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Created Date: 1986-04-24 Completed Date: 1986-04-24 Revised Date: 2005-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 108-15 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Absorption Animals Apolipoprotein A-I Apolipoproteins / metabolism Apolipoproteins A / analysis* Biological Transport Chromatography, Gel Chylomicrons / analysis Glucose / metabolism Lipid Metabolism Lymph / analysis* Male Mesentery / analysis Rats Rats, Inbred Strains Triglycerides / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Apolipoprotein A-I; 0/Apolipoproteins; 0/Apolipoproteins A; 0/Chylomicrons; 0/Triglycerides; 0/apolipoprotein A-IV; 50-99-7/Glucose |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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