Document Detail

Distribution of apolipoproteins A-I and A-IV among lipoprotein classes in rat mesenteric lymph, fractionated by molecular sieve chromatography.
MedLine Citation:
PMID:  3081040     Owner:  NLM     Status:  MEDLINE    
The distribution of apolipoproteins A-I and A-IV among lymph lipoprotein fractions was studied after separation by molecular sieve chromatography, avoiding any ultracentrifugation. Lymph was obtained from rats infused either with a glucose solution or with a triacylglycerol emulsion. Relative to glucose infusion, triacylglycerol infusion caused a 20-fold increase in the output of triacylglycerol, coupled with a 4-fold increase in output of apolipoprotein A-IV. The output of apolipoprotein A-I was only elevated 2-fold. Chromatography on 6% agarose showed that lymph apolipoproteins A-I and A-IV are present on triacylglycerol-rich particles and on particles of the size of HDL. In addition, apolipoprotein A-IV is also present as 'free' apolipoprotein A-IV. The increase in apolipoprotein A-I output is caused by a higher output of A-I associated with large chylomicrons only, while the increase in apolipoprotein A-IV output is reflected by an increased output in all lymph lipoprotein fractions, including lymph HDL and 'free' apolipoprotein A-IV. The increased level of 'free' A-IV, seen in fatty lymph, may contribute to, and at least partly explain, the high concentrations of 'free' apolipoprotein A-IV present in serum obtained from fed animals.
G M Dallinga-Thie; A van Tol; F M van't Hooft; P H Groot
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  876     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  1986 Mar 
Date Detail:
Created Date:  1986-04-24     Completed Date:  1986-04-24     Revised Date:  2005-11-17    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  108-15     Citation Subset:  IM    
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MeSH Terms
Apolipoprotein A-I
Apolipoproteins / metabolism
Apolipoproteins A / analysis*
Biological Transport
Chromatography, Gel
Chylomicrons / analysis
Glucose / metabolism
Lipid Metabolism
Lymph / analysis*
Mesentery / analysis
Rats, Inbred Strains
Triglycerides / metabolism
Reg. No./Substance:
0/Apolipoprotein A-I; 0/Apolipoproteins; 0/Apolipoproteins A; 0/Chylomicrons; 0/Triglycerides; 0/apolipoprotein A-IV; 50-99-7/Glucose

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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