Document Detail


Distribution of alpha1, alpha4, gamma2, and delta subunits of GABAA receptors in hippocampal granule cells.
MedLine Citation:
PMID:  15542076     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
GABAA receptors are pentamers composed of subunits derived from the alpha, beta, gamma, delta, theta, epsilon, and pi gene families. alpha1, alpha4, gamma2, and delta subunits are expressed in the dentate gyrus of the hippocampus, but their subcellular distribution has not been described. Hippocampal sections were double-labeled for the alpha1, alpha4, gamma2, and delta subunits and GAD65 or gephyrin, and their subcellular distribution on dentate granule cells was studied by means of confocal laser scanning microscopy (CLSM). The synaptic versus extrasynaptic localization of these subunits was inferred by quantitative analysis of the frequency of colocalization of various subunits with synaptic markers in high-resolution images. GAD65 immunoreactive clusters colocalized with 26.24+/-0.86% of the alpha1 subunit immunoreactive clusters and 32.35+/-1.49% of the gamma2 subunit clusters. In contrast, only 1.58+/-0.13% of the alpha4 subunit immunoreactive clusters and 1.92+/-0.15% of the delta subunit clusters colocalized with the presynaptic marker GAD65. These findings were confirmed by studying colocalization with immunoreactivity of a postsynaptic marker, gephyrin, which colocalized with 27.61+/-0.16% of the alpha1 subunit immunoreactive clusters and 23.45+/-0.32% of the gamma2 subunit immunoreactive clusters. In contrast, only 1.90+/-0.13% of the alpha4 subunit immunoreactive clusters and 1.76+/-0.10% of the delta subunit clusters colocalized with gephyrin. These studies demonstrate that a subset of alpha1 and gamma2 subunit clusters colocalize with synaptic markers in hippocampal dentate granule cells. Furthermore, all four subunits, alpha1, alpha4, gamma2, and delta, are present in the extrasynaptic locations.
Authors:
Chengsan Sun; Werner Sieghart; Jaideep Kapur
Related Documents :
15306626 - Carboxyl tail region of the kv2.2 subunit mediates novel developmental regulation of ch...
2830346 - Modulation of enterotoxin binding and function in vitro and in vivo.
15102896 - Expression of distinct alpha subunits of gabaa receptor regulates inhibitory synaptic s...
1722386 - Confirmation of the assignment of the vitronectin (vnra) and fibronectin (fnra) recepto...
15278346 - Benzodiazepine receptor and serotonin 2a receptor modulate the aversive-like effects of...
21893106 - Design and characterization of a fluorescent ghrelin analog for imaging the growth horm...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Brain research     Volume:  1029     ISSN:  0006-8993     ISO Abbreviation:  Brain Res.     Publication Date:  2004 Dec 
Date Detail:
Created Date:  2004-11-15     Completed Date:  2005-01-24     Revised Date:  2014-09-20    
Medline Journal Info:
Nlm Unique ID:  0045503     Medline TA:  Brain Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  207-16     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Biological Markers
Carrier Proteins / metabolism
Glutamate Decarboxylase / metabolism
Hippocampus / cytology,  metabolism*
Isoenzymes / metabolism
Male
Membrane Proteins / metabolism
Microscopy, Confocal
Neurons / metabolism*
Protein Subunits / metabolism
Rats
Rats, Sprague-Dawley
Receptors, GABA-A / metabolism*
Synapses / metabolism
Grant Support
ID/Acronym/Agency:
R01 NS040337/NS/NINDS NIH HHS; R01 NS040337-01/NS/NINDS NIH HHS; R01 NS044370/NS/NINDS NIH HHS; R01 NS044370-01A2/NS/NINDS NIH HHS; R01 NS40337/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Carrier Proteins; 0/Isoenzymes; 0/Membrane Proteins; 0/Protein Subunits; 0/Receptors, GABA-A; 0/gephyrin; EC 4.1.1.15/Glutamate Decarboxylase; EC 4.1.1.15/glutamate decarboxylase 2
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Neuroprotective effects of the free radical scavenger Edaravone (MCI-186) in mice permanent focal br...
Next Document:  Evaluation of the tail formalin test in mice as a new model to assess local analgesic effects.