Document Detail

Distribution of Vibrio cholerae virulence genes among different Vibrio species isolated in Sardinia, Italy.
MedLine Citation:
PMID:  10747228     Owner:  NLM     Status:  MEDLINE    
The members of the genus Vibrio include harmless aquatic strains as well as strains capable of causing epidemics of cholera. Diarrhoea caused by Vibrio cholerae is attributed to cholerae enterotoxin (CT) codified by the ctx operon and regulated by a number of virulence genes such as toxT, toxR and toxS. Fifty-two Vibrio strains were isolated from different aquatic environments in and around Sardinia and searched by PCR for the presence of ctxA, zot, ace, toxR, toxS, toxT, tcpA and vpi virulence genes in the genomes of the isolates. The toxR operon was found in 27 Vibrio alginolyticus strains out of 42 analysed, in three out of four V. cholerae non-O1 strains and in three Vibrio parahaemolyticus isolates. A positive amplification for the virulence pathogenic island (vpi) was produced by five V. alginolyticus strains. Finally, the ace expected amplification fragment was found in two V. alginolyticus isolates whereas the amplification with zot primers produced the expected fragment in one V. alginolyticus isolate. Differentiation of these strains with a PCR fingerprinting technique revealed no association between the presence of virulence genes and a particular fingerprinting pattern. Although most Vibrio species are considered non-pathogenic or only potentially harmful to humans, the finding of V. cholerae virulence genes in other members of the genus Vibrio, and the recent reports of the creation and evolution of pandemic strains of V. cholerae, may give a new perspective to the significance of these results.
L A Sechi; I Duprè; A Deriu; G Fadda; S Zanetti
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of applied microbiology     Volume:  88     ISSN:  1364-5072     ISO Abbreviation:  J. Appl. Microbiol.     Publication Date:  2000 Mar 
Date Detail:
Created Date:  2000-05-09     Completed Date:  2000-05-09     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  9706280     Medline TA:  J Appl Microbiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  475-81     Citation Subset:  IM    
Dipartimento di Scienze Biomediche, Sezione di Microbiologia Sperimentale e Clinica, Università degli studi di Sassari, Sassari, Italy.
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MeSH Terms
DNA Fingerprinting
Genes, Bacterial*
Polymerase Chain Reaction
Vibrio / classification,  genetics*,  isolation & purification,  pathogenicity
Vibrio cholerae / genetics*,  pathogenicity*
Virulence / genetics
Water Microbiology*

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