Document Detail


Distribution of Vibrio cholerae virulence genes among different Vibrio species isolated in Sardinia, Italy.
MedLine Citation:
PMID:  10747228     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The members of the genus Vibrio include harmless aquatic strains as well as strains capable of causing epidemics of cholera. Diarrhoea caused by Vibrio cholerae is attributed to cholerae enterotoxin (CT) codified by the ctx operon and regulated by a number of virulence genes such as toxT, toxR and toxS. Fifty-two Vibrio strains were isolated from different aquatic environments in and around Sardinia and searched by PCR for the presence of ctxA, zot, ace, toxR, toxS, toxT, tcpA and vpi virulence genes in the genomes of the isolates. The toxR operon was found in 27 Vibrio alginolyticus strains out of 42 analysed, in three out of four V. cholerae non-O1 strains and in three Vibrio parahaemolyticus isolates. A positive amplification for the virulence pathogenic island (vpi) was produced by five V. alginolyticus strains. Finally, the ace expected amplification fragment was found in two V. alginolyticus isolates whereas the amplification with zot primers produced the expected fragment in one V. alginolyticus isolate. Differentiation of these strains with a PCR fingerprinting technique revealed no association between the presence of virulence genes and a particular fingerprinting pattern. Although most Vibrio species are considered non-pathogenic or only potentially harmful to humans, the finding of V. cholerae virulence genes in other members of the genus Vibrio, and the recent reports of the creation and evolution of pandemic strains of V. cholerae, may give a new perspective to the significance of these results.
Authors:
L A Sechi; I Duprè; A Deriu; G Fadda; S Zanetti
Related Documents :
13783348 - Production of vibrio el tor toxin in a liquid medium.
20132688 - Prevalence of listeria, aeromonas, and vibrio species in fish used for human consumptio...
1588578 - Association of vibrio cholerae with fresh water amoebae.
7911968 - The role of toxin-coregulated pili in the pathogenesis of vibrio cholerae o1 el tor.
18540888 - Kluyveromyces siamensis sp. nov., an ascomycetous yeast isolated from water in a mangro...
18398208 - Rubritalea sabuli sp. nov., a carotenoid- and squalene-producing member of the family v...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of applied microbiology     Volume:  88     ISSN:  1364-5072     ISO Abbreviation:  J. Appl. Microbiol.     Publication Date:  2000 Mar 
Date Detail:
Created Date:  2000-05-09     Completed Date:  2000-05-09     Revised Date:  2000-12-18    
Medline Journal Info:
Nlm Unique ID:  9706280     Medline TA:  J Appl Microbiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  475-81     Citation Subset:  IM    
Affiliation:
Dipartimento di Scienze Biomediche, Sezione di Microbiologia Sperimentale e Clinica, Università degli studi di Sassari, Sassari, Italy. sechila@ssmain.uniss.it
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
DNA Fingerprinting
Genes, Bacterial*
Italy
Phylogeny
Polymerase Chain Reaction
Vibrio / classification,  genetics*,  isolation & purification,  pathogenicity
Vibrio cholerae / genetics*,  pathogenicity*
Virulence / genetics
Water Microbiology*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Microbiological examination of cold ready-to-eat sliced meats from catering establishments in the Un...
Next Document:  Preliminary characterization of bacteriocins produced by Enterococcus faecium and Enterococcus faeca...