Document Detail

Distribution of soluble uranium in the nuclear cell compartment at subtoxic concentrations.
MedLine Citation:
PMID:  21067124     Owner:  NLM     Status:  In-Process    
Uranium is naturally found in the environment, and its extensive use results in an increased risk of human exposure. Kidney cells have mainly been used as in vitro models to study effects of uranium exposure, and very little about the effects on other cell types is known. The aim of this study was to assess the impact of depleted uranium exposure at the cellular level in human kidney (HEK-293), liver (HepG2), and neuronal (IMR-32) cell lines. Cytotoxicity studies showed that these cell lines reacted in a roughly similar manner to depleted uranium exposure, responding at a cytotoxicity threshold of 300-500 μM. Uranium was localized in cells with secondary ion mass spectrometry technology. Results showed that uranium precipitates at subtoxic concentrations (>100 μM). With this approach, we were able for the first time to observe the soluble form of uranium in the cell at low concentrations (10-100 μM). Moreover, this technique allows us to localize it mainly in the nucleus. These innovative results raise the question of how uranium penetrates into cells and open new perspectives for studying the mechanisms of uranium chemical toxicity.
Caroline Rouas; Helene Bensoussan; David Suhard; Christine Tessier; Line Grandcolas; François Rebiere; Isabelle Dublineau; Mohammed Taouis; Marc Pallardy; Philippe Lestaevel; Yann Gueguen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Chemical research in toxicology     Volume:  23     ISSN:  1520-5010     ISO Abbreviation:  Chem. Res. Toxicol.     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2011-12-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8807448     Medline TA:  Chem Res Toxicol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1883-9     Citation Subset:  IM    
Institut de RadioProtection et de Sureté Nucléaire, Direction de la Radioprotection de l’Homme, Service de Radiobiologie et d’Epidémiologie, Laboratoire de Radiotoxicologie Expérimentale, BP17, F-92262 Fontenay-aux-Roses, France.
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