Document Detail


Distribution of Langerhans cells and mast cells within the human oral mucosa: new application sites of allergens in sublingual immunotherapy?
MedLine Citation:
PMID:  18445186     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Sublingual immunotherapy (SLIT) represents an alternative to subcutaneous immunotherapy. While antigen-presenting cells such as Langerhans cells (LCs) are thought to contribute to the effectiveness of SLIT, mast cells (MCs) most likely account for adverse reactions such as sublingual edema. As little is known about LCs and MCs within the oral cavity, we investigated their distribution in search for mucosal sites with highest LCs and lowest MCs density. METHODS: Biopsies were taken simultaneously from human vestibulum, bucca, palatum, lingua, sublingua, gingiva, and skin. Immunohistochemistry and flow cytometry were used to detect MCs, LCs and high affinity receptor for IgE (FcepsilonRI) expression of LCs. Mixed lymphocyte reactions were performed to assess their stimulatory capacity. RESULTS: Highest density of MCs was detected within the gingiva, while the lowest density of MCs was found within the palatum and lingua. However, sublingual MCs were located within glands, which might explain swelling of sublingual caruncle in some SLIT patients. Highest density of LCs was detected within the vestibular region with lowest density in sublingual region. Highest expression of FcepsilonRI was detected on LCs within the vestibulum. Furthermore LCs from different regions displayed similar stimulatory capacity towards allogeneic T cells. CONCLUSIONS: In view of our data, different mucosal regions such as the vestibulum might represent alternative SLIT application sites with potent allergen uptake. Our data might serve as a basis for new application strategies for SLIT to enhance efficiency and reduce local adverse reactions.
Authors:
J-P Allam; G Stojanovski; N Friedrichs; W Peng; T Bieber; J Wenzel; N Novak
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Allergy     Volume:  63     ISSN:  1398-9995     ISO Abbreviation:  Allergy     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-04-30     Completed Date:  2008-07-22     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7804028     Medline TA:  Allergy     Country:  Denmark    
Other Details:
Languages:  eng     Pagination:  720-7     Citation Subset:  IM    
Affiliation:
Department of Dermatology and Allergy, University of Bonn, Bonn, Germany.
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MeSH Terms
Descriptor/Qualifier:
Administration, Sublingual
Cell Count
Desensitization, Immunologic / methods*
Humans
Hypersensitivity / immunology*,  therapy
Langerhans Cells / cytology,  immunology*
Mast Cells / cytology,  immunology*
Mouth Mucosa / cytology,  immunology*
Receptors, IgE / immunology,  metabolism
Chemical
Reg. No./Substance:
0/Receptors, IgE

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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