Document Detail


Distribution of the IgG Fc receptor, FcRn, in the human fetal intestine.
MedLine Citation:
PMID:  12538789     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The intestinal Fc receptor, FcRn, functions in the maternofetal transfer of gamma globulin (IgG) in the neonatal rodent. In humans, most of this transfer is presumed to occur in utero via the placenta. Although the fetus swallows amniotic fluid that contains immunoglobulin, it is unknown whether this transfer also occurs via the fetal intestine. A human FcRn has been identified in the syncytiotrophoblast that mediates the maternofetal transfer of antibody. It has also been identified in human fetal intestine and is postulated to function in IgG transport. We hypothesize that the human fetal intestinal FcRn may play a role in IgG transport from the amniotic fluid into the fetal circulation. The aim of this study was to characterize the distribution of the FcRn along the human fetal intestine. Lysates prepared from human fetal intestine and from a nonmalignant human fetal intestinal epithelial cell line (H4) were subjected to Western blot analysis and probed using anti-FcRn antibodies. A 42-kD band, consistent with the known molecular weight of the FcRn, was detected along the human fetal intestine and in H4 cells. Expression of the human FcRn was confirmed with immunohistochemistry. Our study demonstrates the expression of FcRn along the human fetal intestine and in a human nonmalignant fetal intestinal epithelial cell line (H4), which by location indicates that FcRn could play a role in the uptake and transport of IgG in the human fetus.
Authors:
Uzma Shah; Bonny L Dickinson; Richard S Blumberg; Neil E Simister; Wayne I Lencer; W Allan Walker
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Pediatric research     Volume:  53     ISSN:  0031-3998     ISO Abbreviation:  Pediatr. Res.     Publication Date:  2003 Feb 
Date Detail:
Created Date:  2003-01-22     Completed Date:  2003-09-02     Revised Date:  2014-09-17    
Medline Journal Info:
Nlm Unique ID:  0100714     Medline TA:  Pediatr Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  295-301     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adult
Blotting, Western
Caco-2 Cells / chemistry
Cell Line
Child
Child, Preschool
Colon / cytology,  embryology,  immunology,  metabolism
Epithelial Cells / immunology,  metabolism
Fetus / immunology*,  metabolism*
Humans
Ileum / cytology,  embryology,  immunology,  metabolism
Immunoglobulin G / metabolism*
Immunohistochemistry
Intestinal Mucosa / cytology,  immunology,  metabolism
Intestines / chemistry*,  embryology
Receptors, Fc / immunology,  metabolism*
Stomach / cytology,  embryology,  immunology,  metabolism
Grant Support
ID/Acronym/Agency:
P01 DK033506/DK/NIDDK NIH HHS; P01 DK033506-140001/DK/NIDDK NIH HHS; P01-DK 33506/DK/NIDDK NIH HHS; P30 DK040561/DK/NIDDK NIH HHS; P30 DK040561-06/DK/NIDDK NIH HHS; P30-DK40561/DK/NIDDK NIH HHS; R01 HD031852/HD/NICHD NIH HHS; R01 HD031852-06/HD/NICHD NIH HHS; R01-HD 31852/HD/NICHD NIH HHS; R37 HD012437/HD/NICHD NIH HHS; R37 HD012437-20/HD/NICHD NIH HHS; R37-HD12437/HD/NICHD NIH HHS; T32 DK007477/DK/NIDDK NIH HHS; T32 DK007477-15/DK/NIDDK NIH HHS; T32-DK07477/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Immunoglobulin G; 0/Receptors, Fc
Comments/Corrections

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