Document Detail


Distribution of genes encoding four pathogenicity islands (VPaIs), T6SS, biofilm, and type I pilus in food and clinical strains of Vibrio parahaemolyticus in China.
MedLine Citation:
PMID:  20132020     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Vibrio parahaemolyticus is a major cause of foodborne gastroenteritis in China, Japan, and other countries. The pandemic O3:K6 clone, which harbors thermostable direct hemolysin [tdh] gene and toxRS/new gene, is mainly responsible for the foodborne outbreaks after 1995. Previous studies showed that genes in the pathogenicity island-1 (VPaI-1) and VPaI-5 are harbored only by pandemic strains, whereas genes in VPaI-7 and type III secretion system 2 are closely associated with tdh-positive strains of V. parahaemolyticus. In this study, we examined the distribution of genes encoding VPaI-2, VPaI-3, VPaI-4, VPaI-6, type VI secretion systems (T6SS), biofilm, and type I pilus in 71 food and 116 clinical strains of V. parahaemolyticus. The results showed that most of the pandemic strains of V. parahaemolyticus harbored the complete genes of VPaI-2, T6SS, and type I pilus. In contrast, most of the pathogenic strains (harboring tdh gene or TDH-related hemolysin [trh] gene) and nonpathogenic strains (harboring neither tdh gene nor trh gene) contained partial genes of VPaI-2, T6SS, and type I pilus. Genes of VPaI-4 were exclusively present in the pandemic strains. Genes of VPaI-3 were present in most of the pandemic strains and a small percentage of nonpathogenic strains, mainly O3:K6 strains. VPaI-6 and biofilm-associated genes were harbored by almost all the strains, irrespective of their pandemic, pathogenic, or nonpathogenic traits.
Authors:
Guoxiang Chao; Xinan Jiao; Xiaohui Zhou; Fang Wang; Zhenquan Yang; Jinlin Huang; Zhiming Pan; Liping Zhou; Xiaoqin Qian
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Foodborne pathogens and disease     Volume:  7     ISSN:  1556-7125     ISO Abbreviation:  Foodborne Pathog. Dis.     Publication Date:  2010 Jun 
Date Detail:
Created Date:  2010-06-03     Completed Date:  2010-08-31     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101120121     Medline TA:  Foodborne Pathog Dis     Country:  United States    
Other Details:
Languages:  eng     Pagination:  649-58     Citation Subset:  IM    
Affiliation:
Jiangsu Key Laboratory of Zoonosis, Yangzhou University, Yangzhou, China.
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MeSH Terms
Descriptor/Qualifier:
Bacterial Toxins / genetics,  metabolism
Biofilms*
China / epidemiology
Disease Outbreaks
Fimbriae, Bacterial / genetics*
Food Microbiology*
Foodborne Diseases / prevention & control
Genes, Bacterial
Genomic Islands*
Hemolysin Proteins / genetics,  metabolism
Humans
Polymerase Chain Reaction
Seafood / microbiology
Secretory Pathway*
Species Specificity
Vibrio Infections / epidemiology,  microbiology*
Vibrio parahaemolyticus / genetics*,  isolation & purification,  pathogenicity
Virulence / genetics
Virulence Factors / genetics,  metabolism
Chemical
Reg. No./Substance:
0/Bacterial Toxins; 0/Hemolysin Proteins; 0/Virulence Factors; 135433-21-5/thermostable direct hemolysin

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