Document Detail

Distribution and dynamics of rat basophilic leukemia immunoglobulin E receptors (FcepsilonRI) on planar ligand-presenting surfaces.
MedLine Citation:
PMID:  20643056     Owner:  NLM     Status:  MEDLINE    
There is considerable interest in the signaling mechanisms of immunoreceptors, especially when triggered with membrane-bound ligands. We have quantified the spatiotemporal dynamics of the redistribution of immunoglobulin E-loaded receptors (IgE-FcepsilonRI) on rat basophilic leukemia-2H3 mast cells in contact with fluid and gel-phase membranes displaying ligands for immunoglobulin E, using total internal reflection fluorescence microscopy. To clearly separate the kinetics of receptor redistribution from cell spreading, and to precisely define the initial contact time (+/-50 ms), micropipette cell manipulation was used to bring individual cells into contact with surfaces. On ligand-free surfaces, there are micron-scale heterogeneities in fluorescence that likely reflect regions of the cell that are more closely apposed to the substrate. When ligands are present, receptor clusters form with this same size scale. The initial rate of accumulation of receptors into the clusters is consistent with diffusion-limited trapping with D approximately 10(-1) microm2/s. These results support the hypothesis that clusters form by diffusion to cell-surface contact regions. Over longer timescales (>10 s), individual clusters moved with both diffusive and directed motion components. The dynamics of the cluster motion is similar to the dynamics of membrane fluctuations of cells on ligand-free fluid membranes. Thus, the same cellular machinery may be responsible for both processes.
Kathrin Spendier; Amanda Carroll-Portillo; Keith A Lidke; Bridget S Wilson; Jerilyn A Timlin; James L Thomas
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Biophysical journal     Volume:  99     ISSN:  1542-0086     ISO Abbreviation:  Biophys. J.     Publication Date:  2010 Jul 
Date Detail:
Created Date:  2010-07-20     Completed Date:  2010-10-27     Revised Date:  2014-09-10    
Medline Journal Info:
Nlm Unique ID:  0370626     Medline TA:  Biophys J     Country:  United States    
Other Details:
Languages:  eng     Pagination:  388-97     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2010 Biophysical Society. Published by Elsevier Inc. All rights reserved.
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MeSH Terms
1,2-Dipalmitoylphosphatidylcholine / metabolism*
Cell Line, Tumor
Cell Membrane / metabolism*
Leukemia, Basophilic, Acute / metabolism*,  pathology*
Phosphatidylcholines / metabolism*
Receptors, IgE / metabolism*
Time Factors
Grant Support
P30 CA118100/CA/NCI NIH HHS; R01 AI051575/AI/NIAID NIH HHS; R01 AI051575-08/AI/NIAID NIH HHS
Reg. No./Substance:
0/Ligands; 0/Phosphatidylcholines; 0/Receptors, IgE; 2644-64-6/1,2-Dipalmitoylphosphatidylcholine; TE895536Y5/1-palmitoyl-2-oleoylphosphatidylcholine

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