| Distribution of C22-, C24- and C26-alpha-unit-containing mycolic acid homologues in mycobacteria. | |
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MedLine Citation:
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PMID: 7494494 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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There are three mycolic acid homologues with C22-, C24- and C26-alpha-units in Mycobacterium. In order to reveal the composition and distribution of these homologues in each subclass and molecular species of mycolic acids and to compare them with the composition of constitutive non-polar fatty acids (free and bound forms), we have separated non-polar fatty acids and each subclass of mycolic acids from 21 mycobacterial species by thin-layer chromatography, and analyzed non-polar fatty acid methyl esters by gas chromatography (GC) and the cleavage products of methyl mycolate by pyrolysis GC. We further performed mass chromatographic analysis of trimethylsilyl (TMS) ether derivatives of mycolic acid methyl esters by monitoring [B-29]+ ions (loss of CHO from the alpha-branched-chain structure of mycolic acids) of m/z 426, 454 and 482 which are attributed to C22-, C24- and C26-alpha-units of TMS ether derivatives of methyl mycolates, respectively, (Kaneda, K. et al, J. Clin. Microbiol. 24: 1060-1070, 1986). By pyrolysis GC, C22:0, C24:0 and C26:0 fatty acid methyl esters generated by the C2-C3 cleavage of C22-, C24- and C26-alpha-unit-containing mycolic acid methyl esters, respectively, were detected. Their proportion was almost the same among subclasses of mycolic acids in every Mycobacterium and also similar to the proportion of constitutive non-polar C22:0, C24:0 and C26:0 fatty acids. By mass chromatography, the composition and distribution of C22- and C24-alpha-unit-containing homologues were revealed to be similar between alpha- and alpha'-mycolic acids in every Mycobacterium.(ABSTRACT TRUNCATED AT 250 WORDS) |
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Authors:
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K Kaneda; S Imaizumi; I Yano |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Microbiology and immunology Volume: 39 ISSN: 0385-5600 ISO Abbreviation: Microbiol. Immunol. Publication Date: 1995 |
Date Detail:
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Created Date: 1996-01-11 Completed Date: 1996-01-11 Revised Date: 2000-12-18 |
Medline Journal Info:
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Nlm Unique ID: 7703966 Medline TA: Microbiol Immunol Country: JAPAN |
Other Details:
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Languages: eng Pagination: 563-70 Citation Subset: IM |
Affiliation:
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Department of Bacteriology, Niigata University School of Medicine, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Chromatography, Thin Layer Mycobacterium / chemistry*, genetics Mycolic Acids / analysis*, chemistry Species Specificity |
| Chemical | |
Reg. No./Substance:
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0/Mycolic Acids |
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