Document Detail


Distribution of 2-aminofluorene and p-aminobenzoic acid N-acetyltransferase activity in tissues of C57BL/6J rapid and B6.A-NatS slow acetylator congenic mice.
MedLine Citation:
PMID:  7905384     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The distribution of N-acetyltransferase (NAT) activity in 35 tissues of inbred rapid acetylator C57BL/6J and slow acetylator congenic B6.A-NatS mice was determined by incubation of tissue cytosols with 2-aminofluorene or p-aminobenzoic acid followed by HPLC assay. Tissues examined included the gastrointestinal tract, lymphoid tissues, skin, blood components, and other major organs. NAT activity was found in all tissues examined except blood plasma and seminal vesicles. Peyer's patches had the highest activity with either substrate, and lymphoid tissue, in general, was high in NAT activity as was skin and much of the digestive system. The acetylator polymorphism was apparent in most tissues for both p-aminobenzoic acid and 2-aminofluorene. The difference between rapid and slow acetylator phenotypes was usually greater with p-aminobenzoic acid than with 2-aminofluorene. The presence of NAT in the 33 tissues of rapid and slow acetylator mice, as well as the absence of NAT in plasma and seminal vesicles, was confirmed by immunoblots using an anti-NAT antibody raised in rabbits. These results indicate the widespread distribution of NAT activity and the relative abundance of extrahepatic N-acetylation capacity in the mouse.
Authors:
J G Chung; G N Levy; W W Weber
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Drug metabolism and disposition: the biological fate of chemicals     Volume:  21     ISSN:  0090-9556     ISO Abbreviation:  Drug Metab. Dispos.     Publication Date:    1993 Nov-Dec
Date Detail:
Created Date:  1994-03-04     Completed Date:  1994-03-04     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9421550     Medline TA:  Drug Metab Dispos     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1057-63     Citation Subset:  IM    
Affiliation:
Department of Pharmacology, University of Michigan, Ann Arbor 48109-0626.
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MeSH Terms
Descriptor/Qualifier:
4-Aminobenzoic Acid / metabolism*
Acetylation
Alleles
Animals
Arylamine N-Acetyltransferase / blood,  genetics,  metabolism*
Cytosol / enzymology
Digestive System / enzymology
Electrophoresis
Fluorenes / metabolism*
Lymphoid Tissue / enzymology
Male
Mice
Mice, Inbred C57BL
Mice, Inbred Strains
Seminal Vesicles / enzymology
Tissue Distribution
Grant Support
ID/Acronym/Agency:
CA 39018/CA/NCI NIH HHS; OH 00081/OH/NIOSH CDC HHS
Chemical
Reg. No./Substance:
0/Fluorenes; 150-13-0/4-Aminobenzoic Acid; 153-78-6/2-aminofluorene; EC 2.3.1.5/Arylamine N-Acetyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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