Document Detail

Distinguishing direct from indirect roles for bicoid mRNA localization factors.
MedLine Citation:
PMID:  20023172     Owner:  NLM     Status:  MEDLINE    
Localization of bicoid mRNA to the anterior of the Drosophila oocyte is essential for patterning the anteroposterior body axis in the early embryo. bicoid mRNA localizes in a complex multistep process involving transacting factors, molecular motors and cytoskeletal components that remodel extensively during the lifetime of the mRNA. Genetic requirements for several localization factors, including Swallow and Staufen, are well established, but the precise roles of these factors and their relationship to bicoid mRNA transport particles remains unresolved. Here we use live cell imaging, super-resolution microscopy in fixed cells and immunoelectron microscopy on ultrathin frozen sections to study the distribution of Swallow, Staufen, actin and dynein relative to bicoid mRNA during late oogenesis. We show that Swallow and bicoid mRNA are transported independently and are not colocalized at their final destination. Furthermore, Swallow is not required for bicoid transport. Instead, Swallow localizes to the oocyte plasma membrane, in close proximity to actin filaments, and we present evidence that Swallow functions during the late phase of bicoid localization by regulating the actin cytoskeleton. In contrast, Staufen, dynein and bicoid mRNA form nonmembranous, electron dense particles at the oocyte anterior. Our results exclude a role for Swallow in linking bicoid mRNA to the dynein motor. Instead we propose a model for bicoid mRNA localization in which Swallow is transported independently by dynein and contributes indirectly to bicoid mRNA localization by organizing the cytoskeleton, whereas Staufen plays a direct role in dynein-dependent bicoid mRNA transport.
Timothy T Weil; Despina Xanthakis; Richard Parton; Ian Dobbie; Catherine Rabouille; Elizabeth R Gavis; Ilan Davis
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Development (Cambridge, England)     Volume:  137     ISSN:  1477-9129     ISO Abbreviation:  Development     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2009-12-21     Completed Date:  2010-01-27     Revised Date:  2014-09-16    
Medline Journal Info:
Nlm Unique ID:  8701744     Medline TA:  Development     Country:  England    
Other Details:
Languages:  eng     Pagination:  169-76     Citation Subset:  IM    
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MeSH Terms
Actins / genetics,  metabolism,  physiology*
Cell Membrane / metabolism,  ultrastructure
Drosophila Proteins / genetics,  metabolism,  physiology*
Dyneins / genetics,  physiology*
Embryo, Nonmammalian / metabolism,  ultrastructure
Gene Expression Regulation, Developmental / genetics,  physiology
Homeodomain Proteins / genetics*
In Situ Hybridization
Microscopy, Fluorescence
Microscopy, Immunoelectron
Oocytes / metabolism,  ultrastructure
Oogenesis / genetics,  physiology
RNA, Messenger / metabolism*
RNA-Binding Proteins / genetics,  metabolism,  physiology*
Trans-Activators / genetics*
Grant Support
081858//Wellcome Trust; GM067758/GM/NIGMS NIH HHS; R01 GM067758/GM/NIGMS NIH HHS
Reg. No./Substance:
0/Actins; 0/Drosophila Proteins; 0/Homeodomain Proteins; 0/RNA, Messenger; 0/RNA-Binding Proteins; 0/Trans-Activators; 0/bicoid protein, Drosophila; 0/swallow protein, Drosophila; 139568-71-1/stau protein, Drosophila; EC

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