Document Detail

Distinctive effects of rat fibroblast growth factor-2 isoforms on PC12 and Schwann cells.
MedLine Citation:
PMID:  11811791     Owner:  NLM     Status:  MEDLINE    
Fibroblast growth factor-2 (FGF-2) is an important modulator of cell growth and differentiation and stimulates cell survival of various cells including neurons. Rat FGF-2 occurs in three isoforms, a low molecular weight 18 kD and two high molecular weight forms (21, 23 kD), representing alternative translation products from a single mRNA. The 18 kD isoform shows mainly cytoplasmatic localization, whereas the 21/23 kD FGF-2 are localized in the nucleus. In addition, the FGF-2 isoforms are differentially regulated in the sensory ganglia and peripheral nerve following nerve injury and in the adrenal medulla during post-natal development and after hormonal stimuli. The distinct intracellular distribution and differential regulation of the different FGF-2 isoforms indicate that they have unique biological roles, however, little is known about the biological effects of the high molecular weight FGF-2 isoforms. Immortalized Schwann cells and PC12 cells, which stably overexpress the different FGF-2 isoforms, showed that the different endogenous-overexpressed FGF-2 isoforms lead to dramatic modifications in cell proliferation and survival, when tested in serum-free and serum-containing medium. In contrast, application of recombinant FGF-2 isoforms on normal PC12 and immortalized Schwann cells results in similar biological effects on the proliferation and survival of the cells. Furthermore, we investigated the potential regulatory effects of endogenous-overexpressed and exogenous-applied FGF-2 isoforms on the mRNA level of the FGF-2 receptors and, additionally, on the tyrosin hydroxylase mRNA expression in PC12 cells.
F Müller-Ostermeyer; P Claus; C Grothe
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Growth factors (Chur, Switzerland)     Volume:  19     ISSN:  0897-7194     ISO Abbreviation:  Growth Factors     Publication Date:  2001  
Date Detail:
Created Date:  2002-01-28     Completed Date:  2002-07-22     Revised Date:  2012-06-05    
Medline Journal Info:
Nlm Unique ID:  9000468     Medline TA:  Growth Factors     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  175-91     Citation Subset:  IM    
Hannover Medical School, Center of Anatomy, Germany.
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MeSH Terms
Cell Division
Cell Survival
Fibroblast Growth Factor 2 / chemistry*
Fibroblasts / metabolism
PC12 Cells
Plasmids / metabolism
Protein Binding
Protein Isoforms
Protein-Tyrosine Kinases*
RNA, Messenger / metabolism
Receptor Protein-Tyrosine Kinases / biosynthesis
Receptor, Fibroblast Growth Factor, Type 1
Receptor, Fibroblast Growth Factor, Type 3
Receptor, Fibroblast Growth Factor, Type 4
Receptors, Fibroblast Growth Factor / biosynthesis
Recombinant Proteins / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Schwann Cells / metabolism
Reg. No./Substance:
0/Protein Isoforms; 0/RNA, Messenger; 0/Receptors, Fibroblast Growth Factor; 0/Recombinant Proteins; 103107-01-3/Fibroblast Growth Factor 2; EC protein, rat; EC protein, rat; EC Kinases; EC Protein-Tyrosine Kinases; EC, Fibroblast Growth Factor, Type 1; EC, Fibroblast Growth Factor, Type 3; EC, Fibroblast Growth Factor, Type 4

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