| Distinctive effects of rat fibroblast growth factor-2 isoforms on PC12 and Schwann cells. | |
| | |
MedLine Citation:
|
PMID: 11811791 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Fibroblast growth factor-2 (FGF-2) is an important modulator of cell growth and differentiation and stimulates cell survival of various cells including neurons. Rat FGF-2 occurs in three isoforms, a low molecular weight 18 kD and two high molecular weight forms (21, 23 kD), representing alternative translation products from a single mRNA. The 18 kD isoform shows mainly cytoplasmatic localization, whereas the 21/23 kD FGF-2 are localized in the nucleus. In addition, the FGF-2 isoforms are differentially regulated in the sensory ganglia and peripheral nerve following nerve injury and in the adrenal medulla during post-natal development and after hormonal stimuli. The distinct intracellular distribution and differential regulation of the different FGF-2 isoforms indicate that they have unique biological roles, however, little is known about the biological effects of the high molecular weight FGF-2 isoforms. Immortalized Schwann cells and PC12 cells, which stably overexpress the different FGF-2 isoforms, showed that the different endogenous-overexpressed FGF-2 isoforms lead to dramatic modifications in cell proliferation and survival, when tested in serum-free and serum-containing medium. In contrast, application of recombinant FGF-2 isoforms on normal PC12 and immortalized Schwann cells results in similar biological effects on the proliferation and survival of the cells. Furthermore, we investigated the potential regulatory effects of endogenous-overexpressed and exogenous-applied FGF-2 isoforms on the mRNA level of the FGF-2 receptors and, additionally, on the tyrosin hydroxylase mRNA expression in PC12 cells. |
| | |
Authors:
|
F Müller-Ostermeyer; P Claus; C Grothe |
Publication Detail:
|
Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Growth factors (Chur, Switzerland) Volume: 19 ISSN: 0897-7194 ISO Abbreviation: Growth Factors Publication Date: 2001 |
Date Detail:
|
Created Date: 2002-01-28 Completed Date: 2002-07-22 Revised Date: 2012-06-05 |
Medline Journal Info:
|
Nlm Unique ID: 9000468 Medline TA: Growth Factors Country: Switzerland |
Other Details:
|
Languages: eng Pagination: 175-91 Citation Subset: IM |
Affiliation:
|
Hannover Medical School, Center of Anatomy, Germany. mueller-ostermeyer.frauke@mh-hannover.de |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Cell Division Cell Survival Fibroblast Growth Factor 2 / chemistry* Fibroblasts / metabolism PC12 Cells Plasmids / metabolism Protein Binding Protein Isoforms Protein-Tyrosine Kinases* RNA, Messenger / metabolism Rats Receptor Protein-Tyrosine Kinases / biosynthesis Receptor, Fibroblast Growth Factor, Type 1 Receptor, Fibroblast Growth Factor, Type 3 Receptor, Fibroblast Growth Factor, Type 4 Receptors, Fibroblast Growth Factor / biosynthesis Recombinant Proteins / metabolism Reverse Transcriptase Polymerase Chain Reaction Schwann Cells / metabolism Transfection |
| Chemical | |
Reg. No./Substance:
|
0/Protein Isoforms; 0/RNA, Messenger; 0/Receptors, Fibroblast Growth Factor; 0/Recombinant Proteins; 103107-01-3/Fibroblast Growth Factor 2; EC 2.7.10.1/Fgfr1 protein, rat; EC 2.7.10.1/Fgfr4 protein, rat; EC 2.7.10.1/Protein-Tyrosine Kinases; EC 2.7.10.1/Receptor Protein-Tyrosine Kinases; EC 2.7.10.1/Receptor, Fibroblast Growth Factor, Type 1; EC 2.7.10.1/Receptor, Fibroblast Growth Factor, Type 3; EC 2.7.10.1/Receptor, Fibroblast Growth Factor, Type 4 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Contribution of residues A54 and L55 of the human insulin-like growth factor-II (IGF-II) A domain to...
Next Document: Effect of placenta growth factor-1 on proliferation and release of nitric oxide, cyclic AMP and cycl...