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Distinction of two different classes of small-cell lung cancer cell lines by enzymatically inactive neuron-specific enolase.
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MedLine Citation:
PMID:  1333786     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Neuron specific enolase (NSE) is widely used as a neuro-endocrine marker. However the presence of NSE in many non-neuroendocrine tissues has raised questions on the specificity of NSE. We have investigated NSE immunoreactivity (NSA-ag), gamma-enolase activity and total enolase activity in small cell lung cancer (SCLC) cell lines. During well-controlled exponential growth comparison of NSE-ag content and gamma-enolase activity with the doubling-time (Td) and NSE-ag content with gamma-enolase and total enolase activity led to a clear distinction of two types of cell line: variant cell lines plus part of the classic cell lines (type I) and the remaining classic cell lines (type II). The distinction was based upon both an abrupt 6-fold increase of gamma-enolase activity and an 18-fold increase of NSE-ag, which for the larger part was enzymatically inactive. Within each group the increase of NSE-ag content was significantly correlated with the increase of gamma-enolase activity and both NSE-ag content and gamma-enolase activity increased linearly with Td. It is concluded that gamma-enolase seems to be associated with the regulation of growth rate and that a compound with the gamma-enolase antigen but without enzyme activity can distinguish two different classes of SCLC cell lines. Furthermore the demonstration that NSE-ag can represent the active enzyme as well as an enzymatically inactive compound may explain why a controversy about neuron- or non-specificity of NSE exists.
Authors:
T A Splinter; C F Verkoelen; M Vlastuin; T C Kok; G Rijksen; K G Haglid; F Boomsma; A van de Gaast
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  British journal of cancer     Volume:  66     ISSN:  0007-0920     ISO Abbreviation:  Br. J. Cancer     Publication Date:  1992 Dec 
Date Detail:
Created Date:  1993-01-14     Completed Date:  1993-01-14     Revised Date:  2010-09-07    
Medline Journal Info:
Nlm Unique ID:  0370635     Medline TA:  Br J Cancer     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  1065-9     Citation Subset:  IM    
Affiliation:
Department of Medical Oncology, University Hospital, Rotterdam, The Netherlands.
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MeSH Terms
Descriptor/Qualifier:
Carcinoma, Small Cell / classification,  enzymology*,  pathology
Cell Division
Humans
Phosphopyruvate Hydratase / analysis*
Tumor Cells, Cultured / enzymology,  pathology
Chemical
Reg. No./Substance:
EC 4.2.1.11/Phosphopyruvate Hydratase
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): Br J Cancer
ISSN: 0007-0920
ISSN: 1532-1827
Article Information
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Print publication date: Month: 12 Year: 1992
Volume: 66 Issue: 6
First Page: 1065 Last Page: 1069
ID: 1978033
PubMed Id: 1333786

Distinction of two different classes of small-cell lung cancer cell lines by enzymatically inactive neuron-specific enolase.
T. A. Splinter
C. F. Verkoelen
M. Vlastuin
T. C. Kok
G. Rijksen
K. G. Haglid
F. Boomsma
A. van de Gaast
Department of Medical Oncology, University Hospital, Rotterdam, The Netherlands.



Article Categories:
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