| Distinct subpopulations of IgG memory B cells respond to different molecular forms of the same hapten. | |
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MedLine Citation:
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PMID: 357659 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Spleen cells from mice primed with the thymus-dependent antigen trinitrophenyl keyhold limpet hemocyanin several months earlier can be cultured in vitro to give vigorous IgG antihapten PFC responses to thymus-dependent (TD) and thymus-independent (TI) forms of the same hapten. Here we show that the IgG memory precursors that respond to these two forms of the hapten constitute functionally distinct subpopulations. We have designated these subpopulations as B1gamma and B2gamma to represent secondary precursor cells responding to TI and TD antigens, respectively. Three types of evidence for these subpopulations are presented: 1) In vitro secondary IgG responses to TD and TI forms of the TNP hapten are additive when both forms are added to the same culture. 2) The precursor frequencies for the TD and TI antigens are additive, but addition is not observed between two TD or two TI antigens. 3) Each population can be selectively eliminated by BUdR and light treatment without affecting the other population. The ontogenetic relationships between these subpopulations are discussed in relation to all presently proposed subpopulations B1mu, B2mu, B1gamma, and B2gamma. |
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Authors:
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T V Tittle; M B Rittenberg |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of immunology (Baltimore, Md. : 1950) Volume: 121 ISSN: 0022-1767 ISO Abbreviation: J. Immunol. Publication Date: 1978 Sep |
Date Detail:
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Created Date: 1978-11-18 Completed Date: 1978-11-18 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985117R Medline TA: J Immunol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 936-41 Citation Subset: AIM; IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals B-Lymphocytes / immunology* Bromodeoxyuridine / pharmacology Cell Separation Clone Cells / immunology Female Haptens* Hemocyanin / immunology* Hemolytic Plaque Technique Immunoglobulin G* Immunologic Memory* Light Mice Mice, Inbred BALB C Thymus Gland / immunology Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Haptens; 0/Immunoglobulin G; 59-14-3/Bromodeoxyuridine; 9013-72-3/Hemocyanin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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