Document Detail

Distinct regulation of inner medullary collecting duct nitric oxide production from mice and rats.
MedLine Citation:
PMID:  23331097     Owner:  NLM     Status:  MEDLINE    
Nitric oxide (NO) and NO synthase 1 (NOS1) maintain sodium and water homeostasis. The NOS1α and NOS1β splice variants are expressed in the rat inner medulla, but only NOS1β is expressed in the mouse. Collecting duct NOS1 is necessary for blood pressure control. We hypothesized that NOS1 splice variant expression and NO production in the inner medullary collecting duct (IMCD) are regulated differently in mice and rats by high dietary sodium. Male C57blk/J6 mice and Sprague-Dawley rats were fed a 0.4% (normal salt; NS), or 4% (high salt; HS) NaCl diet for 2 or 7 days. Mean arterial pressure was not altered by HS, whereas urinary sodium excretion in mice and rats was increased significantly. Urinary excretion of nitrate/nitrite (NO(x)) and IMCD nitrite production were significantly greater in mice compared with rats on the HS diet. Western blotting indicated that only NOS1β and NOS3 were expressed in the mouse IMCD and that expression was unaffected by the HS diet at either time point. In contrast, NOS1α was detected in the IMCD of rats, in addition to NOS1β and NOS3. Feeding of the HS diet for 2 days increased NOS1α and NOS1β expression in the rat IMCD and 7 day feeding of the HS diet further increased NOS1β expression. Expression of NOS3 was unchanged by the HS diet at either time point. In conclusion, IMCD NO production in mice and rats is distinctly regulated under both NS and HS conditions, including expression of NOS1 splice variants.
Kelly A Hyndman; Jing Xue; Alexander MacDonell; Joshua S Speed; Chunhua Jin; Jennifer S Pollock
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Clinical and experimental pharmacology & physiology     Volume:  40     ISSN:  1440-1681     ISO Abbreviation:  Clin. Exp. Pharmacol. Physiol.     Publication Date:  2013 Mar 
Date Detail:
Created Date:  2013-02-26     Completed Date:  2013-08-16     Revised Date:  2014-03-06    
Medline Journal Info:
Nlm Unique ID:  0425076     Medline TA:  Clin Exp Pharmacol Physiol     Country:  Australia    
Other Details:
Languages:  eng     Pagination:  233-9     Citation Subset:  IM    
Copyright Information:
© 2013 The Authors Clinical and Experimental Pharmacology and Physiology © 2013 Wiley Publishing Asia Pty Ltd.
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MeSH Terms
Arterial Pressure / drug effects
Blotting, Western
Kidney Medulla / drug effects,  enzymology*,  metabolism
Kidney Tubules, Collecting / drug effects,  enzymology*,  metabolism
Mice, Inbred C57BL
Nitrates / urine
Nitric Oxide / biosynthesis*
Nitric Oxide Synthase Type I / biosynthesis*
Nitrites / urine
Protein Isoforms
Rats, Sprague-Dawley
Sodium Chloride, Dietary / administration & dosage*,  pharmacology
Species Specificity
Grant Support
Reg. No./Substance:
0/Nitrates; 0/Nitrites; 0/Protein Isoforms; 0/Sodium Chloride, Dietary; 31C4KY9ESH/Nitric Oxide; EC Oxide Synthase Type I; EC protein, mouse; EC protein, rat

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