Document Detail

Distinct protease pathways control cell shape and apoptosis in v-src-transformed quail neuroretina cells.
MedLine Citation:
PMID:  16202997     Owner:  NLM     Status:  MEDLINE    
Intracellular proteases play key roles in cell differentiation, proliferation and apoptosis. In nerve cells, little is known about their relative contribution to the pathways which control cell physiology, including cell death. Neoplastic transformation of avian neuroretina cells by p60(v-src) tyrosine kinase results in dramatic morphological changes and deregulation of apoptosis. To identify the proteases involved in the cellular response to p60(v-src), we evaluated the effect of specific inhibitors of caspases, calpains and the proteasome on cell shape changes and apoptosis induced by p60(v-src) inactivation in quail neuroretina cells transformed by tsNY68, a thermosensitive strain of Rous sarcoma virus. We found that the ubiquitin-proteasome pathway is recruited early after p60(v-src) inactivation and is critical for morphological changes, whereas caspases are essential for cell death. This study provides evidence that distinct intracellular proteases are involved in the control of the morphology and fate of v-src-transformed cells.
Benjamin D Néel; Abdel Aouacheria; Anne-Laure Nouvion; Xavier Ronot; Germain Gillet
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-10-03
Journal Detail:
Title:  Experimental cell research     Volume:  311     ISSN:  0014-4827     ISO Abbreviation:  Exp. Cell Res.     Publication Date:  2005 Nov 
Date Detail:
Created Date:  2005-10-31     Completed Date:  2005-12-22     Revised Date:  2009-11-19    
Medline Journal Info:
Nlm Unique ID:  0373226     Medline TA:  Exp Cell Res     Country:  United States    
Other Details:
Languages:  eng     Pagination:  106-16     Citation Subset:  IM    
IBCP, UMR 5086 CNRS/Université Claude Bernard, IFR 128, 7 passage du Vercors, F69367, Lyon cedex 07, France.
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MeSH Terms
Avian Sarcoma Viruses / physiology
Blotting, Western
Calpain / antagonists & inhibitors,  metabolism
Caspases / antagonists & inhibitors,  physiology*
Cell Shape*
Cell Transformation, Viral*
Cells, Cultured
Cysteine Proteinase Inhibitors / pharmacology
Flow Cytometry
Microscopy, Phase-Contrast
Oncogene Protein pp60(v-src) / physiology*
Proteasome Endopeptidase Complex / antagonists & inhibitors,  metabolism
Retina / embryology,  pathology*,  virology
Signal Transduction / physiology*
Ubiquitin / metabolism
Reg. No./Substance:
0/Cysteine Proteinase Inhibitors; 0/Ubiquitin; EC Protein pp60(v-src); EC 3.4.22.-/Calpain; EC 3.4.22.-/Caspases; EC Endopeptidase Complex

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