Document Detail

Distinct patterns of expression of the D-type cyclins during testicular development in the mouse.
MedLine Citation:
PMID:  7537638     Owner:  NLM     Status:  MEDLINE    
The three D-type cyclins have been shown to be differentially expressed in a number of isolated cell types and cell lines, suggesting distinct roles in cell cycle regulation in particular cell lineages. The testis provides unique opportunities to study genes involved in cell cycle regulation, since it contains cells in both mitosis and meiosis as well as differentiated cells with little proliferation activity. Major transcripts of 4.2 kb, 6.8 kb, and 2.3 kb were detected in the adult mouse testis by Northern hybridization analyses for cyclin D1, cyclin D2, and cyclin D3, respectively. Additional transcripts of 1.8 and 2.7 kb were detected by Northern hybridization for cyclin D3 in the testis, but not in other tissues, and these transcripts were limited to germ cells. Northern and in situ hybridization analyses of normal and germ cell-deficient testes showed the surprising result that cyclin D1 was expressed in a pattern consistent with expression in the non-dividing Sertoli cells. Cyclin D2 levels appeared slightly enriched in germ cell-deficient testes as compared to intact testis, but in situ hybridization analysis did not reveal any distinct cellular localization. Also surprising was the observation that cyclin D3 expression was highest in the non-dividing, haploid, round spermatids. The possible roles of these cyclins in the events of spermatogenesis are discussed.
S E Ravnik; K Rhee; D J Wolgemuth
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Developmental genetics     Volume:  16     ISSN:  0192-253X     ISO Abbreviation:  Dev. Genet.     Publication Date:  1995  
Date Detail:
Created Date:  1995-06-06     Completed Date:  1995-06-06     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7909963     Medline TA:  Dev Genet     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  171-8     Citation Subset:  IM; S    
Department of Genetics and Development, Columbia University College of Physicians and Surgeons, New York, NY 10032, USA.
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MeSH Terms
Cell Line
Cyclins / genetics*
Gene Expression Regulation, Developmental*
RNA / genetics,  metabolism
Testis / cytology,  embryology*,  metabolism
Grant Support
Reg. No./Substance:
0/Cyclins; 63231-63-0/RNA

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