| Distinct pathways for modification of the bacterial cell wall by non-canonical D-amino acids. | |
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MedLine Citation:
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PMID: 21792174 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Production of non-canonical D-amino acids (NCDAAs) in stationary phase promotes remodelling of peptidoglycan (PG), the polymer that comprises the bacterial cell wall. Impairment of NCDAAs production leads to excessive accumulation of PG and hypersensitivity to osmotic shock; however, the mechanistic bases for these phenotypes were not previously determined. Here, we show that incorporation of NCDAAs into PG is a critical means by which NCDAAs control PG abundance and strength. We identified and reconstituted in vitro two (of at least three) distinct processes that mediate NCDAA incorporation. Diverse bacterial phyla incorporate NCDAAs into their cell walls, either through periplasmic editing of the mature PG or via incorporation into PG precursor subunits in the cytosol. Production of NCDAAs in Vibrio cholerae requires the stress response sigma factor RpoS, suggesting that NCDAAs may aid bacteria in responding to varied environmental challenges. The widespread capacity of diverse bacteria, including non-producers, to incorporate NCDAAs suggests that these amino acids may serve as both autocrine- and paracrine-like regulators of chemical and physical properties of the cell wall in microbial communities. |
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Authors:
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Felipe Cava; Miguel A de Pedro; Hubert Lam; Brigid M Davis; Matthew K Waldor |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2011-07-26 |
Journal Detail:
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Title: The EMBO journal Volume: 30 ISSN: 1460-2075 ISO Abbreviation: EMBO J. Publication Date: 2011 Aug |
Date Detail:
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Created Date: 2011-08-17 Completed Date: 2011-11-02 Revised Date: 2011-12-22 |
Medline Journal Info:
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Nlm Unique ID: 8208664 Medline TA: EMBO J Country: England |
Other Details:
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Languages: eng Pagination: 3442-53 Citation Subset: IM |
Affiliation:
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Department of Microbiology and Molecular Genetics, Channing Laboratory, Brigham and Women's Hospital, Harvard Medical School and HHMI, Boston, MA, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Amino Acids / chemistry, metabolism*, pharmacology* Bacterial Proteins / genetics, metabolism Base Sequence Cell Wall / metabolism* Membrane Proteins / genetics, metabolism* Models, Molecular Molecular Conformation Molecular Sequence Data Peptide Fragments / metabolism Peptide Synthases / genetics, metabolism Peptidoglycan / biosynthesis, drug effects* Peptidyl Transferases / genetics, metabolism Sequence Alignment Sequence Homology, Amino Acid Stereoisomerism Structure-Activity Relationship Substrate Specificity Vibrio cholerae / genetics, metabolism, ultrastructure |
| Grant Support | |
ID/Acronym/Agency:
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AI-R37-42347/AI/NIAID NIH HHS; R37 AI042347-17/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Bacterial Proteins; 0/Membrane Proteins; 0/Peptide Fragments; 0/Peptidoglycan; EC 2.3.2.12/Peptidyl Transferases; EC 6.3.2.-/Peptide Synthases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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