Document Detail


Distinct organization of energy metabolism in HL-1 cardiac cell line and cardiomyocytes.
MedLine Citation:
PMID:  18423391     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Expression and function of creatine kinase (CK), adenylate kinase (AK) and hexokinase (HK) isoforms in relation to their roles in regulation of oxidative phosphorylation (OXPHOS) and intracellular energy transfer were assessed in beating (B) and non-beating (NB) cardiac HL-l cell lines and adult rat cardiomyocytes or myocardium. In both types of HL-1 cells, the AK2, CKB, HK1 and HK2 genes were expressed at higher levels than the CKM, CKMT2 and AK1 genes. Contrary to the saponin-permeabilized cardiomyocytes the OXPHOS was coupled to mitochondrial AK and HK but not to mitochondrial CK, and neither direct transfer of adenine nucleotides between CaMgATPases and mitochondria nor functional coupling between CK-MM and CaMgATPases was observed in permeabilized HL-1 cells. The HL-1 cells also exhibited deficient complex I of the respiratory chain. In conclusion, contrary to cardiomyocytes where mitochondria and CaMgATPases are organized into tight complexes which ensure effective energy transfer and feedback signaling between these structures via specialized pathways mediated by CK and AK isoforms and direct adenine nucleotide channeling, these complexes do not exist in HL-1 cells due to less organized energy metabolism.
Authors:
Margus Eimre; Kalju Paju; Sophie Pelloux; Nathalie Beraud; Mart Roosimaa; Lumme Kadaja; Marju Gruno; Nadezhda Peet; Ehte Orlova; Reele Remmelkoor; Andres Piirsoo; Valdur Saks; Enn Seppet
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-03-29
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1777     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2008 Jun 
Date Detail:
Created Date:  2008-06-09     Completed Date:  2008-07-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  514-24     Citation Subset:  IM    
Affiliation:
Department of Pathophysiology, Centre of Molecular and Clinical Medicine, Faculty of Medicine, University of Tartu, Tartu, Estonia.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line
Isoenzymes / metabolism
Male
Mitochondria, Heart / enzymology*
Muscle Proteins / metabolism*
Myocardium / enzymology*
Myocytes, Cardiac / enzymology*
Oxidative Phosphorylation*
Rats
Rats, Wistar
Chemical
Reg. No./Substance:
0/Isoenzymes; 0/Muscle Proteins

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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