| Distinct neutrophil subpopulations phenotype by flow cytometry in myelodysplastic syndromes. | |
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MedLine Citation:
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PMID: 19294559 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The cardinal feature of myelodysplastic syndromes (MDS) is dysplasia involving one or more myeloid cell lineages. In the present study, we used 4-color flow cytometric analysis to investigate dysgranulopoiesis in bone marrow specimens from 65 patients with MDS. The antigen expression patterns of total neutrophil granulocytes (TNG) and of the two distinct neutrophil granulocytic subpopulations (NGSs), NGS-1 (dimmer CD45 expression) and NGS-2 (stronger CD45 expression) identified on the side scatter (SS) vs. CD45-intensity plot, were studied. The neutrophil granulocytes from patients with MDS showed characteristic antigen expression aberrancies which were more pronounced in NGS-2 subpopulation. Studying separately the NGS-2 subpopulation with the CD16/MPO/LF combination, the low CD16(+)/MPO(+) and low CD16(+)/LF(+) percentages seemed to discriminate between lower-risk and higher-risk patients with MDS in most occasions. Furthermore, a detailed assessment of the NGS-1 and NGS-2 immunophenotypic patterns revealed early dysplastic changes, not otherwise observed by standard TNG analysis, especially in cases of lower-risk MDS. |
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Authors:
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Myrofora Vikentiou; Katerina Psarra; Violetta Kapsimali; Konstantinos Liapis; Michalis Michael; Konstantinos Tsionos; Evi Lianidou; Chryssa Papasteriades |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Leukemia & lymphoma Volume: 50 ISSN: 1029-2403 ISO Abbreviation: Leuk. Lymphoma Publication Date: 2009 Mar |
Date Detail:
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Created Date: 2009-04-06 Completed Date: 2009-08-17 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9007422 Medline TA: Leuk Lymphoma Country: England |
Other Details:
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Languages: eng Pagination: 401-9 Citation Subset: IM |
Affiliation:
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Department of Chemistry, National and Kapodistrian University of Athens, Athens, Greece. mvikentiou@biol.uoa.gr |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Antigens, CD45
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analysis Antigens, Surface / analysis Bone Marrow / pathology Flow Cytometry / methods* Humans Immunophenotyping Myelodysplastic Syndromes / diagnosis, pathology* Neutrophils / pathology* Receptors, IgG Risk Assessment |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Surface; 0/Receptors, IgG; EC 3.1.3.48/Antigens, CD45 |
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