Document Detail


Distinct microbial populations exist in the mucosa-associated microbiota of sub-groups of irritable bowel syndrome.
MedLine Citation:
PMID:  22070725     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Background  There is increasing evidence to support a role for the gastrointestinal microbiota in the etiology of irritable bowel syndrome (IBS). Given the evidence of an inflammatory component to IBS, the mucosa-associated microbiota potentially play a key role in its pathogenesis. The objectives were to compare the mucosa-associated microbiota between patients with diarrhea predominant IBS (IBS-D), constipation predominant IBS (IBS-C) and controls using fluorescent in situ hybridization and to correlate specific bacteria groups with individual IBS symptoms. Methods  Forty-seven patients with IBS (27 IBS-D and 20 IBS-C) and 26 healthy controls were recruited to the study. Snap-frozen rectal biopsies were taken at colonoscopy and bacterial quantification performed by hybridizing frozen sections with bacterial-group specific oligonucleotide probes. Key Results  Patients with IBS had significantly greater numbers of total mucosa-associated bacteria per mm of rectal epithelium than controls [median 218 (IQR - 209) vs 128 (121) P = 0.007], and this was chiefly comprised of bacteroides IBS [69 (67) vs 14 (41) P = 0.001] and Eubacterium rectale-Clostridium coccoides [52 (58) vs 25 (35) P = 0.03]. Analysis of IBS sub-groups demonstrated that bifidobacteria were lower in the IBS-D group than in the IBS-C group and controls [24 (32) vs 54 (88) vs 32 (35) P = 0.011]. Finally, amongst patients with IBS, the maximum number of stools per day negatively correlated with the number of mucosa-associated bifidobacteria (P < 0.001) and lactobacilli (P = 0.002). Conclusions & Inferences  The mucosa-associated microbiota in patients with IBS is significantly different from healthy controls with increases in bacteroides and clostridia and a reduction in bifidobacteria in patients with IBS-D.
Authors:
G C Parkes; N B Rayment; B N Hudspith; L Petrovska; M C Lomer; J Brostoff; K Whelan; J D Sanderson
Related Documents :
6553245 - Biofeedback training for reduced respiratory rate in chronic obstructive pulmonary dise...
1670225 - Impact of transhiatal esophagectomy on cardiac and respiratory function.
8045145 - Nasal mechanical ventilation for hypercapnic respiratory failure in chronic obstructive...
1994965 - New approaches in the rehabilitation of the traumatic high level quadriplegic.
21979995 - Use of implantable loop recorders in patients with brugada syndrome and suspected risk ...
2858665 - Pathophysiological and prognostic significance of cerebrospinal-fluid lactate in cerebr...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-11-9
Journal Detail:
Title:  Neurogastroenterology and motility : the official journal of the European Gastrointestinal Motility Society     Volume:  -     ISSN:  1365-2982     ISO Abbreviation:  -     Publication Date:  2011 Nov 
Date Detail:
Created Date:  2011-11-10     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9432572     Medline TA:  Neurogastroenterol Motil     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2011 Blackwell Publishing Ltd.
Affiliation:
Diet and Gastrointestinal Health, Nutritional Sciences Division, King's College London, London, UK Department of Gastroenterology, Guy's and St Thomas' NHS Foundation Trust, London, UK.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  The role of protamine amount in the transfection performance of cationic SLN designed as a gene nano...
Next Document:  Evidence-based practice in speech-language pathology curricula: A scoping study.