Document Detail


Distinct mesenchymal progenitor cell subsets in the adult human synovium.
MedLine Citation:
PMID:  19605375     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: To analyse the heterogeneity at the single-cell level of human mesenchymal progenitor cells from SM.
METHODS: Cell populations were enzymatically released from the knee joint synovium of adult human individuals. Single cell-derived clonal populations were obtained by limiting dilution and serially passaged to determine growth rates. Phenotypic analysis was carried out by flow cytometry. Replicative senescence was assessed by the senescence-associated beta-galactosidase staining. Telomere lengths were determined semiquantitatively by Southern blotting. Telomerase activity was measured using a real-time quantitative telomerase repeat amplification procedure. Culture-expanded clonal populations were subjected to in vitro differentiation assays to investigate their mesenchymal multipotency.
RESULTS: The 50 clones analysed displayed wide variations in the proliferation rates, even within the same donor sample. The time taken to reach 20 population doublings ranged from 44 to 130 days. The phenotype of the clones tested was compatible with that of mesenchymal stem cells. Mean telomere lengths ranged from 5.2 to 10.9 kb with positive linear trend with telomerase activity, but no correlation with proliferative rates or cell senescence. All clones tested were capable of chondrogenic and osteogenic differentiation, though with large variability in potency. In contrast, only 30% of the clones were adipogenic.
CONCLUSIONS: We report for the first time the co-existence, within the synovium, of progenitor cell subsets with distinct mesenchymal differentiation potency. Our findings further emphasize the need for strategies to purify cell populations with the clinically desired tissue formation potentials.
Authors:
Alexandra Karystinou; Francesco Dell'Accio; Tobias B A Kurth; Henning Wackerhage; Ilyas M Khan; Charles W Archer; Elena A Jones; Thimios A Mitsiadis; Cosimo De Bari
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Publication Detail:
Type:  Journal Article     Date:  2009-07-14
Journal Detail:
Title:  Rheumatology (Oxford, England)     Volume:  48     ISSN:  1462-0332     ISO Abbreviation:  Rheumatology (Oxford)     Publication Date:  2009 Sep 
Date Detail:
Created Date:  2009-08-18     Completed Date:  2009-10-09     Revised Date:  2014-02-19    
Medline Journal Info:
Nlm Unique ID:  100883501     Medline TA:  Rheumatology (Oxford)     Country:  England    
Other Details:
Languages:  eng     Pagination:  1057-64     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Aged
Aged, 80 and over
Cell Aging / physiology
Cell Division / physiology
Cells, Cultured
Chondrogenesis / physiology
Female
Humans
Immunophenotyping
Knee Joint / cytology
Male
Mesenchymal Stromal Cells / cytology*
Middle Aged
Osteogenesis / physiology
Synovial Membrane / cytology*
Telomerase / metabolism
Telomere / ultrastructure
Grant Support
ID/Acronym/Agency:
G108/620//Medical Research Council
Chemical
Reg. No./Substance:
EC 2.7.7.49/Telomerase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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