Document Detail


Distinct immune response induced by peptidoglycan derived from Lactobacillus sp.
MedLine Citation:
PMID:  16419162     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIM: To analyze the distinct immune responses induced by Lactobacillus peptidoglycan (PG). METHODS: BALB/c mice were intraperitoneally injected with PG once a day for three consecutive days. Peritoneal macrophage and splenocyte mRNA was extracted and the gene expression profile was studied using high-density oligonucleotide microarrays. Inhibitory effects of Lactobacillus PG on colon tumor tissue were studied in vitro and in vivo. RESULTS: The gene expression profiles revealed that the TLR-NF-kappaB; and Jak-STAT signaling pathways were highly activated. An inflammatory phenotype was induced when peritoneal macrophages were initially exposed to Lactobacillus PG and switched to a more complex phenotype when BALB/c mice were treated with three doses of Lactobacillus PG. A protective physiological inflammatory response was induced after three consecutive days of PG treatment. It was tending toward Th1 dominant immune response. Lactobacillus PG also appeared to induce a significant in vivo anti-colon tumor effect. CONCLUSION: Lactobacillus PG is responsible for certain immune responses induced by Lactobacilli. Anti-tumor effects of Lactobacilli are likely to attribute to the activation of macrophages by PG expressed on the bacterial cell surface.
Authors:
Jin Sun; Yong-Hui Shi; Guo-Wei Le; Xi-Yi Ma
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  World journal of gastroenterology : WJG     Volume:  11     ISSN:  1007-9327     ISO Abbreviation:  World J. Gastroenterol.     Publication Date:  2005 Oct 
Date Detail:
Created Date:  2006-01-18     Completed Date:  2006-03-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  100883448     Medline TA:  World J Gastroenterol     Country:  China    
Other Details:
Languages:  eng     Pagination:  6330-7     Citation Subset:  IM    
Affiliation:
Institute of Food Nutrition and Safety, School of Food Science and Technology, Southern Yangtze University, Wuxi 214036, Jiangsu Province, China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Line, Tumor
Colonic Neoplasms / genetics,  metabolism
Gene Expression Profiling
Immune System / physiology*
Inflammation / immunology
Lactobacillus / immunology*
Macrophages, Peritoneal / immunology
Mice
Mice, Inbred BALB C
Oligonucleotide Array Sequence Analysis
Peptidoglycan / immunology*
Phenotype
Signal Transduction / physiology
Spleen / cytology,  immunology
Chemical
Reg. No./Substance:
0/Peptidoglycan

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