| Distinct epigenetic regulation of tumor suppressor genes in putative cancer stem cells of solid tumors. | |
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MedLine Citation:
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PMID: 21042723 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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Epigenetic gene regulation plays essential roles in differentiation of embryonic and tissue stem cells. In these benign undifferentiated cells, some polycomb targeted genes are kept in a state of DNA hypomethylation and they have a distinct chromatin signature termed bivalent chromatin structure to maintain their plasticity. We hypothesized that cancer stem cells (CSC), the malignant counterpart of these cells, are also under the control of epigenetics like benign stem cells. We compared the DNA methylation and chromatin structure in 10 tumor suppressor genes between CSC and differentiated cancer cells of MCF7 and Huh7 cells. We found that the level of DNA methylation was indeed significantly lower in CSC, while surprisingly, the bivalent chromatin structure was more ubiquitously seen in differentiated cancer cells compared to CSC. However, repressive marks of chromatin structure, namely H3K27me3 and EZH2, were significantly lower in CSC. As a consequence, CSC remained in a higher transcriptionally active chromatin state compared to differentiated cancer cells. We found that the differentiation of CSCs is also epigenetically regulated. These findings could help towards a comprehensive understanding of CSC, and also improve the development of eradicative therapies against human malignancies. |
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Authors:
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Hiroyuki Yasuda; Kenzo Soejima; Hideo Watanabe; Ichiro Kawada; Ichiro Nakachi; Satoshi Yoda; Sohei Nakayama; Ryosuke Satomi; Shinnosuke Ikemura; Hideki Terai; Takashi Sato; Sadafumi Suzuki; Yumi Matsuzaki; Katsuhiko Naoki; Akitoshi Ishizaka |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: International journal of oncology Volume: 37 ISSN: 1791-2423 ISO Abbreviation: Int. J. Oncol. Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-11-02 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9306042 Medline TA: Int J Oncol Country: Greece |
Other Details:
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Languages: eng Pagination: 1537-46 Citation Subset: IM |
Affiliation:
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Department of Pulmonary Medicine, School of Medicine, Keio University, Shinjuku-ku, Tokyo 160-8582, Japan. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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