Document Detail

Distinct cytoskeletal modulation and regulation of G1-S transition in the two life stages of Trypanosoma brucei.
MedLine Citation:
PMID:  16144864     Owner:  NLM     Status:  MEDLINE    
Procyclic-form Trypanosoma brucei is arrested in G1 phase with extended and/or branched posterior morphology when expression of its cdc2-related kinases 1 and 2 (CRK1 and CRK2) is knocked down by RNA interference. Transmission electron microscopy indicated that the mitochondrion in the cell is also extended and branched and associated with cortical microtubules in each elongated/branched posterior end. This posterior extension is apparently driven by the growing microtubule corset, as it can be blocked by rhizoxin, an inhibitor of microtubule assembly. In the bloodstream form of T. brucei, however, a knockdown of CRK1 and CRK2 resulted only in an enrichment of cells in G1 phase without cessation of DNA synthesis or elongated/branched posterior ends. A triple knockdown of CRK1, CRK2 and CycE1/CYC2 in the bloodstream form resulted in 15% of the cells arrested in G1 phase, but no cells had an abnormal posterior morphology. The double and triple knockdown bloodstream-form cells were differentiated in vitro into the procyclic form, and the latter thus generated bore the typical morphology of a procyclic form without an extended/branched posterior end, albeit arrested in the G1 phase as the bloodstream-form precursor. There is thus a major distinction in the mechanisms regulating G1-S transition and posterior morphogenesis between the two life stages of T. brucei.
Xiaoming Tu; Joel Mancuso; W Zacheus Cande; Ching C Wang
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, P.H.S.     Date:  2005-09-06
Journal Detail:
Title:  Journal of cell science     Volume:  118     ISSN:  0021-9533     ISO Abbreviation:  J. Cell. Sci.     Publication Date:  2005 Oct 
Date Detail:
Created Date:  2005-09-23     Completed Date:  2006-01-27     Revised Date:  2012-06-15    
Medline Journal Info:
Nlm Unique ID:  0052457     Medline TA:  J Cell Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  4353-64     Citation Subset:  IM    
Department of Pharmaceutical Chemistry, University of California, 600 16th Street, San Francisco, CA 94143-2280, USA.
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MeSH Terms
CDC2 Protein Kinase / genetics,  metabolism
Cell Shape
Cells, Cultured
Cyclins / genetics,  metabolism
Cytoskeleton / drug effects,  metabolism*
DNA / biosynthesis
Fluorescent Dyes / metabolism
G1 Phase / physiology*
Life Cycle Stages
Macrolides / pharmacology
Microtubules / drug effects,  metabolism
Mitochondria / metabolism,  ultrastructure
Protozoan Proteins / genetics,  metabolism
RNA Interference
S Phase / physiology*
Trypanosoma brucei brucei / drug effects,  physiology*,  ultrastructure
Tubulin Modulators / pharmacology
Grant Support
Reg. No./Substance:
0/Cyclins; 0/Fluorescent Dyes; 0/Macrolides; 0/Protozoan Proteins; 0/Tubulin Modulators; 0/cyc2 protein, Paramecium tetraurelia; 9007-49-2/DNA; 90996-54-6/rhizoxin; EC Protein Kinase; EC protein,Trypanosoma brucei; EC protein, Trypanosoma brucei

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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