Document Detail


Distinct cutaneous bacterial assemblages in a sampling of South American Amerindians and US residents.
MedLine Citation:
PMID:  22895161     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The human skin harbors complex bacterial communities. Prior studies showing high inter-individual variation focused on subjects from developed countries. We therefore compared cutaneous bacterial communities of Amerindians in the Venezuelan Amazon with subjects in the United States. Forearm skin specimens were studied from healthy Amerindians in Platanillal village in Amazonas State, and from healthy persons in New York and Colorado. All skin sampling used similar swab/buffer techniques. Multiplexed V2-targeted 16S rRNA gene pyrosequencing yielded high quality sequences from 112 samples. The results show 20 phyla, with three (Proteobacteria, Firmicutes, Actinobacteria) predominating. US residents and Venezuelan Amerindians had significantly different forearm skin bacterial community compositions, with United States dominated by Propionibacterium. Among the Amerindians, there was a deep split based on bacterial community membership, with 30 and 42 samples, respectively, falling into each of the two groups, not associated with age, gender, or body mass index. One Amerindian group had diversity similar to the United States, but was dominated by Staphylococcus rather than Propionibacterium. The other Amerindian group was significantly more diverse and even than the US or the other Amerindian group, and featured a broad range of Proteobacteria. The results provide evidence that ethnicity, lifestyle and/or geography are associated with the structure of human cutaneous bacterial communities.
Authors:
Martin J Blaser; Maria G Dominguez-Bello; Monica Contreras; Magda Magris; Glida Hidalgo; Isidoro Estrada; Zhan Gao; Jose C Clemente; Elizabeth K Costello; Rob Knight
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-08-16
Journal Detail:
Title:  The ISME journal     Volume:  7     ISSN:  1751-7370     ISO Abbreviation:  ISME J     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2012-12-18     Completed Date:  2013-08-30     Revised Date:  2014-01-09    
Medline Journal Info:
Nlm Unique ID:  101301086     Medline TA:  ISME J     Country:  England    
Other Details:
Languages:  eng     Pagination:  85-95     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adult
Bacteria / classification*,  genetics,  isolation & purification*
Biodiversity
Colorado
DNA, Bacterial / genetics
Feces / parasitology
Female
Humans
Male
Metagenome*
Middle Aged
New York
Phylogeny
Propionibacterium / genetics,  isolation & purification
Proteobacteria / genetics,  isolation & purification
RNA, Ribosomal, 16S / genetics
Skin / microbiology*
Staphylococcus / genetics,  isolation & purification
Venezuela
Grant Support
ID/Acronym/Agency:
DK090989/DK/NIDDK NIH HHS; HG004866/HG/NHGRI NIH HHS; HG004872/HG/NHGRI NIH HHS; UH2 AR057056/AR/NIAMS NIH HHS; //Howard Hughes Medical Institute
Chemical
Reg. No./Substance:
0/DNA, Bacterial; 0/RNA, Ribosomal, 16S
Comments/Corrections

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