Document Detail


Distinct ceramide synthases regulate polarized growth in the filamentous fungus Aspergillus nidulans.
MedLine Citation:
PMID:  16394102     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In filamentous fungi, the stabilization of a polarity axis is likely to be a pivotal event underlying the emergence of a germ tube from a germinating spore. Recent results implicate the polarisome in this process and also suggest that it requires localized membrane organization. Here, we employ a chemical genetic approach to demonstrate that ceramide synthesis is necessary for the formation of a stable polarity axis in the model fungus Aspergillus nidulans. We demonstrate that a novel compound (HSAF) produced by a bacterial biocontrol agent disrupts polarized growth and leads to loss of membrane organization and formin localization at hyphal tips. We show that BarA, a putative acyl-CoA-dependent ceramide synthase that is unique to filamentous fungi mediates the effects of HSAF. Moreover, A. nidulans possesses a second likely ceramide synthase that is essential and also regulates hyphal morphogenesis. Our results suggest that filamentous fungi possess distinct pools of ceramide that make independent contributions to polarized hyphal growth, perhaps through the formation of specialized lipid microdomains that regulate organization of the cytoskeleton.
Authors:
Shaojie Li; Liangcheng Du; Gary Yuen; Steven D Harris
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-01-04
Journal Detail:
Title:  Molecular biology of the cell     Volume:  17     ISSN:  1059-1524     ISO Abbreviation:  Mol. Biol. Cell     Publication Date:  2006 Mar 
Date Detail:
Created Date:  2006-02-27     Completed Date:  2006-06-19     Revised Date:  2013-06-07    
Medline Journal Info:
Nlm Unique ID:  9201390     Medline TA:  Mol Biol Cell     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1218-27     Citation Subset:  IM    
Affiliation:
Department of Plant Pathology, University of Nebraska, Lincoln, NE 68588-0660, USA.
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MeSH Terms
Descriptor/Qualifier:
Acyl Coenzyme A / metabolism
Antifungal Agents / metabolism
Aspergillus nidulans / cytology*,  enzymology*,  growth & development
Cell Polarity*
Drug Resistance, Fungal
Fungal Proteins / metabolism
Hyphae / cytology
Mixed Function Oxygenases / metabolism
Models, Biological
Mutation / genetics
Oxidoreductases / metabolism*
Phenotype
Phylogeny
Saccharomyces cerevisiae Proteins / metabolism
Sphingolipids / deficiency,  metabolism
Chemical
Reg. No./Substance:
0/Acyl Coenzyme A; 0/Antifungal Agents; 0/Fungal Proteins; 0/Saccharomyces cerevisiae Proteins; 0/Sphingolipids; EC 1.-/Mixed Function Oxygenases; EC 1.-/Oxidoreductases; EC 1.14.-/SUR2 protein, S cerevisiae; EC 1.3.1.-/dihydroceramide desaturase
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